| pubmed-article:16675174 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16675174 | lifeskim:mentions | umls-concept:C0019158 | lld:lifeskim |
| pubmed-article:16675174 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
| pubmed-article:16675174 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:16675174 | lifeskim:mentions | umls-concept:C0699749 | lld:lifeskim |
| pubmed-article:16675174 | lifeskim:mentions | umls-concept:C0011209 | lld:lifeskim |
| pubmed-article:16675174 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:16675174 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:16675174 | pubmed:dateCreated | 2006-7-4 | lld:pubmed |
| pubmed-article:16675174 | pubmed:abstractText | In order to determine the influence of hepatic disease-stage on polyethylenimine-mediated gene delivery, we investigated branched and linear polyethylenimine (B-PEI, L-PEI)-mediated plasmid DNA delivery with time in murine hepatitis induced by a subcutaneous injection of tetrachloro carbon (CCl(4)). Plasmid DNA (pDNA) encoding firefly luciferase was used as the model reporter gene. We determined luciferase activity in various organs of CCl(4)-treated mice and control mice after an intravenous administration of B-PEI and L-PEI/pDNA complexes. Both B-PEI and L-PEI/pDNA complexes showed significantly lower gene expression in the liver, spleen, and lung at the stage of severe hepatitis (18 h after CCl(4) injection), whereas the complexes induced gene expression in the liver at the liver regeneration stage (48 h after CCl(4) injection). Significant differences in gene expressions between CCl(4)-treated mice and control mice vanished in most organs at the hepatitis subsidence stage (168 h after CCl(4) injection), indicating that the influence of hepatitis induced by CCl(4) was reversible with PEI-mediated gene delivery. Our findings demonstrated that murine hepatitis induced by CCl(4) could influence polyethylenimine-mediated plasmid DNA delivery according to the disease stage. These results indicate the necessity of considering the timing and dose of gene therapy according to the disease stage. | lld:pubmed |
| pubmed-article:16675174 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16675174 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16675174 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16675174 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16675174 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16675174 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16675174 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16675174 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16675174 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16675174 | pubmed:issn | 0378-5173 | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:KitaharaTakas... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:YoshiokaTakas... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:NishidaKoyoK | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:NakashimaMiki... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:SasakiHitoshi... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:NakamuraJunzo... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:NakagawaHiroo... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:YoshidaShohei... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:IchikawaNobuh... | lld:pubmed |
| pubmed-article:16675174 | pubmed:author | pubmed-author:NakamuraTadah... | lld:pubmed |
| pubmed-article:16675174 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16675174 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:16675174 | pubmed:volume | 318 | lld:pubmed |
| pubmed-article:16675174 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16675174 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16675174 | pubmed:pagination | 139-45 | lld:pubmed |
| pubmed-article:16675174 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
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| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
| pubmed-article:16675174 | pubmed:meshHeading | pubmed-meshheading:16675174... | lld:pubmed |
| pubmed-article:16675174 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16675174 | pubmed:articleTitle | Influence of disease stage on polyethylenimine-mediated plasmid DNA delivery in murine hepatitis. | lld:pubmed |
| pubmed-article:16675174 | pubmed:affiliation | Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. sasaki@net.nagasaki-u.ac.jp | lld:pubmed |
| pubmed-article:16675174 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16675174 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
