Source:http://linkedlifedata.com/resource/pubmed/id/16675174
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
2006-7-4
|
| pubmed:abstractText |
In order to determine the influence of hepatic disease-stage on polyethylenimine-mediated gene delivery, we investigated branched and linear polyethylenimine (B-PEI, L-PEI)-mediated plasmid DNA delivery with time in murine hepatitis induced by a subcutaneous injection of tetrachloro carbon (CCl(4)). Plasmid DNA (pDNA) encoding firefly luciferase was used as the model reporter gene. We determined luciferase activity in various organs of CCl(4)-treated mice and control mice after an intravenous administration of B-PEI and L-PEI/pDNA complexes. Both B-PEI and L-PEI/pDNA complexes showed significantly lower gene expression in the liver, spleen, and lung at the stage of severe hepatitis (18 h after CCl(4) injection), whereas the complexes induced gene expression in the liver at the liver regeneration stage (48 h after CCl(4) injection). Significant differences in gene expressions between CCl(4)-treated mice and control mice vanished in most organs at the hepatitis subsidence stage (168 h after CCl(4) injection), indicating that the influence of hepatitis induced by CCl(4) was reversible with PEI-mediated gene delivery. Our findings demonstrated that murine hepatitis induced by CCl(4) could influence polyethylenimine-mediated plasmid DNA delivery according to the disease stage. These results indicate the necessity of considering the timing and dose of gene therapy according to the disease stage.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0378-5173
|
| pubmed:author |
pubmed-author:IchikawaNobuhiroN,
pubmed-author:KitaharaTakashiT,
pubmed-author:NakagawaHirooH,
pubmed-author:NakamuraJunzoJ,
pubmed-author:NakamuraTadahiroT,
pubmed-author:NakashimaMikiroM,
pubmed-author:NishidaKoyoK,
pubmed-author:SasakiHitoshiH,
pubmed-author:YoshidaShoheiS,
pubmed-author:YoshiokaTakashiT
|
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
318
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
139-45
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:16675174-Animals,
pubmed-meshheading:16675174-Carbon Tetrachloride Poisoning,
pubmed-meshheading:16675174-Chromatography, Gel,
pubmed-meshheading:16675174-DNA,
pubmed-meshheading:16675174-Drug-Induced Liver Injury,
pubmed-meshheading:16675174-Excipients,
pubmed-meshheading:16675174-Gene Expression,
pubmed-meshheading:16675174-Gene Therapy,
pubmed-meshheading:16675174-Genes, Reporter,
pubmed-meshheading:16675174-Hepatitis, Animal,
pubmed-meshheading:16675174-Liver,
pubmed-meshheading:16675174-Luciferases,
pubmed-meshheading:16675174-Male,
pubmed-meshheading:16675174-Mice,
pubmed-meshheading:16675174-Plasmids,
pubmed-meshheading:16675174-Polyethyleneimine,
pubmed-meshheading:16675174-Tissue Distribution,
pubmed-meshheading:16675174-Transgenes
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Influence of disease stage on polyethylenimine-mediated plasmid DNA delivery in murine hepatitis.
|
| pubmed:affiliation |
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. sasaki@net.nagasaki-u.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|