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rdf:type |
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lifeskim:mentions |
umls-concept:C0023810,
umls-concept:C0040649,
umls-concept:C0040661,
umls-concept:C0063700,
umls-concept:C0079189,
umls-concept:C0205224,
umls-concept:C0205263,
umls-concept:C0521447,
umls-concept:C1120843,
umls-concept:C1280500,
umls-concept:C1314939,
umls-concept:C1416433,
umls-concept:C1564138,
umls-concept:C1704259,
umls-concept:C1705162,
umls-concept:C1705987,
umls-concept:C1710082
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pubmed:issue |
4
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pubmed:dateCreated |
2006-5-4
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pubmed:abstractText |
Indoleamine 2,3-dioxygenase (IDO) is induced by interferon (IFN)-gamma-mediated effects of the signal transducer and activator of transcription 1alpha (STAT1alpha) and interferon regulatory factor (IRF)-1. The induction of IDO can also be mediated through an IFN-gamma-independent mechanism, although the mechanism of induction has not been identified. In this study, we explored whether lipopolysaccharide (LPS) or several proinflammatory cytokines can induce IDO via an IFN-gamma-independent mechanism, and whether IDO induction by LPS requires the STAT1alpha and IRF-1 signaling pathways. IDO was induced by LPS or IFN-gamma in peripheral blood mononuclear cells and THP-1 cells, and a synergistic IDO induction occurred when THP-1 cells were cultured in the presence of a combination of tumor necrosis factor-alpha, interleukin-6 or interleukin-1beta. An electrophoretic mobility shift assay using STAT1alpha and IRF-1 consensus oligonucleotide probes showed no STAT1alpha or IRF-1 binding activities in LPS-stimulated THP-1 cells. Further, the LPS-induced IDO activity was inhibited by both p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappaB (NF-kappaB) inhibitors. These findings suggest that the induction of IDO by LPS in THP-1 cells is not regulated by IFN-gamma via recruitment of STAT1alpha or IRF-1 to the intracellular signaling pathway, and may be related to the activity of the p38 MAPK pathway and NF-kappaB.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Indoleamine-Pyrrole 2,3,-Dioxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/STAT1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/TNF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-924X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
139
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
655-62
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16672265-Cell Line,
pubmed-meshheading:16672265-Cell Line, Tumor,
pubmed-meshheading:16672265-Cytokines,
pubmed-meshheading:16672265-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16672265-Enzyme Activation,
pubmed-meshheading:16672265-Enzyme Inhibitors,
pubmed-meshheading:16672265-Humans,
pubmed-meshheading:16672265-Indoleamine-Pyrrole 2,3,-Dioxygenase,
pubmed-meshheading:16672265-Interferon Regulatory Factor-1,
pubmed-meshheading:16672265-Interferon-gamma,
pubmed-meshheading:16672265-Lipopolysaccharides,
pubmed-meshheading:16672265-Models, Biological,
pubmed-meshheading:16672265-NF-kappa B,
pubmed-meshheading:16672265-STAT1 Transcription Factor,
pubmed-meshheading:16672265-Tumor Necrosis Factor-alpha,
pubmed-meshheading:16672265-U937 Cells,
pubmed-meshheading:16672265-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2006
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pubmed:articleTitle |
The signal transducer and activator of transcription 1alpha and interferon regulatory factor 1 are not essential for the induction of indoleamine 2,3-dioxygenase by lipopolysaccharide: involvement of p38 mitogen-activated protein kinase and nuclear factor-kappaB pathways, and synergistic effect of several proinflammatory cytokines.
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pubmed:affiliation |
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu City, Gifu 501-1194.
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pubmed:publicationType |
Journal Article
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