Source:http://linkedlifedata.com/resource/pubmed/id/16668372
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
2010-6-29
|
pubmed:abstractText |
Four variants of arcelin, an insecticidal seed storage protein of bean, Phaseolus vulgaris L., were investigated. Each variant (arcelin-1, -2, -3, and -4) was purified, and solubilities and M(r)s were determined. For arcelins-1, -2, and -4, the isoelectric points, hemagglutinating activities, immunological cross-reactivities, and N-terminal amino acid sequences were determined. On the basis of native and denatured M(r)s, the variants were classified as being composed of dimer protein (arcelin-2), tetramer protein (arcelins-3 and -4), or both dimer and tetramer proteins (arcelin-1). Although the dimer proteins (arcelins-1d and -2) could be distinguished by M(r)s and isoelectric points, they were identical for their first 37 N-terminal amino acids and had similar immunological cross-reactions, and bean lines containing these variants had a DNA restriction fragment in common. The tetramer proteins arcelin-1t and arcelin-4 also could be distinguished from each other based on M(r)s and isoelectric points; however, they had similar immunological cross-reactions and they were 77 to 93% identical for N-terminal amino acid composition. The similarities among arcelin variants, phytohemagglutinin, and a bean alpha-amylase inhibitor suggest that they are all encoded by related members of a lectin gene family.
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-1265481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-16665920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-16667338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-1691123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-17800917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-2152175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-2990911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-3611052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-3843705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-4563441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6175244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6424503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6530601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6897567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-861037
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0032-0889
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
97
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
204-11
|
pubmed:dateRevised |
2010-9-14
|
pubmed:year |
1991
|
pubmed:articleTitle |
Characterization and comparison of arcelin seed protein variants from common bean.
|
pubmed:affiliation |
Department of Agronomy, University of Wisconsin, Madison, Wisconsin 53706.
|
pubmed:publicationType |
Journal Article
|