Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Four variants of arcelin, an insecticidal seed storage protein of bean, Phaseolus vulgaris L., were investigated. Each variant (arcelin-1, -2, -3, and -4) was purified, and solubilities and M(r)s were determined. For arcelins-1, -2, and -4, the isoelectric points, hemagglutinating activities, immunological cross-reactivities, and N-terminal amino acid sequences were determined. On the basis of native and denatured M(r)s, the variants were classified as being composed of dimer protein (arcelin-2), tetramer protein (arcelins-3 and -4), or both dimer and tetramer proteins (arcelin-1). Although the dimer proteins (arcelins-1d and -2) could be distinguished by M(r)s and isoelectric points, they were identical for their first 37 N-terminal amino acids and had similar immunological cross-reactions, and bean lines containing these variants had a DNA restriction fragment in common. The tetramer proteins arcelin-1t and arcelin-4 also could be distinguished from each other based on M(r)s and isoelectric points; however, they had similar immunological cross-reactions and they were 77 to 93% identical for N-terminal amino acid composition. The similarities among arcelin variants, phytohemagglutinin, and a bean alpha-amylase inhibitor suggest that they are all encoded by related members of a lectin gene family.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-1265481, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-16665920, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-16667338, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-1691123, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-17800917, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-2152175, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-2990911, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-3611052, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-3843705, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-4563441, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6175244, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6424503, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6530601, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-6897567, http://linkedlifedata.com/resource/pubmed/commentcorrection/16668372-861037
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Sep
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
204-11
pubmed:dateRevised
2010-9-14
pubmed:year
1991
pubmed:articleTitle
Characterization and comparison of arcelin seed protein variants from common bean.
pubmed:affiliation
Department of Agronomy, University of Wisconsin, Madison, Wisconsin 53706.
pubmed:publicationType
Journal Article