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rdf:type |
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lifeskim:mentions |
umls-concept:C0010028,
umls-concept:C0026237,
umls-concept:C0033684,
umls-concept:C0039711,
umls-concept:C0475264,
umls-concept:C1138842,
umls-concept:C1160500,
umls-concept:C1167624,
umls-concept:C1548779,
umls-concept:C1744614,
umls-concept:C1947902,
umls-concept:C1998793,
umls-concept:C2698124,
umls-concept:C2827499
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pubmed:issue |
3
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
The protein T-URF13 (URF13) is specific to mitochondria of maize (Zea mays L.) with Texas (T) male-sterile cytoplasm and has been implicated in causing male sterility and susceptibility to T-cytoplasm-specific fungal diseases. T-URF13 was purified from isolated mitochondria from maize (line B73) with T cytoplasm by gel filtration and a quasi two-dimensional polyacrylamide gel electrophoresis system. Antibodies to the purified and denatured protein were produced in rabbits. Anti-T-URF13 antiserum was used to show that T-URF13 is in the inner membrane of mitochondria and behaves as an integral membrane protein when mitochondria are fractionated with sodium carbonate or Triton X-114. The antiserum and protein A tagged with 20-nanometer-gold particles were used to localize T-URF13 in T mitochondria by electron microscopy of sections of isolated mitochondria from etiolated shoots and sections of roots and of tapetal cells at pre-and post-degeneration stages of microsporogenesis. The microscopic study confirms that T-URF13 is specifically localized in the mitochondrial membranes of all of the T mitochondria tested, notably those in the tapetum from the meiocyte stage to the late-microspore stage. No change in the amount of labeled T-URF13 protein in the mitochondria of aging tapetal cells was detected.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-16592554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-16592757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-16593863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-16665845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-2136632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-2303028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-2417728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-2466658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-2660137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-2885095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-3021525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-3276005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-3534077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-3863132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-4145130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-6190419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-6276012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-6361451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-6361481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-6400651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-6424501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-6502707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668065-7068748
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
861-70
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pubmed:dateRevised |
2010-9-14
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pubmed:year |
1991
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pubmed:articleTitle |
T-URF 13 Protein from Mitochondria of Texas Male-Sterile Maize (Zea mays L.) : Its Purification and Submitochondrial Localization, and Immunogold Labeling in Anther Tapetum during Microsporogenesis.
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pubmed:affiliation |
Department of Botany, Iowa State University, Ames, Iowa 50011-1020.
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pubmed:publicationType |
Journal Article
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