Source:http://linkedlifedata.com/resource/pubmed/id/16666973
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
Aspartate aminotransferase (l-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1 [AAT]), a key enzyme in the assimilation of C and N compounds, was purified from the cytosol of alfalfa (Medicago sativa L.) root nodules. Isoforms that increased during nodule development, AAT-2a, AAT-2b, and AAT-2c, were purified greater than 447-fold to apparent homogeneity, and high titer polyclonal antibodies were produced. The native molecular weight of the AAT-2 isoforms was approximately 80 kilodatons with a subunit molecular weight of 40 kilodatons, indicating that the holoenzymes are dimers. The AAT-2 isoforms comprised approximately 0.4% of the total soluble nodule protein. The AAT specific activity was measured in leaf, stem, root, and nodule organs, and zymograms of each were compared. Enzyme activity was 4- to 37-fold greater in effective (nitrogen fixing) nodules than in leaves, stems, and roots. Effective nodule AAT-specific activity was 3- to 8-fold greater than that of plant-controlled ineffective nodules. No differences in K(m) were observed between AAT-1 and AAT-2. Antibodies raised against AAT-2 were more selective against AAT-2 than AAT-1. Evidence obtained from zymograms suggests that the expression of alfalfa nodule AAT is controlled at two different gene loci, AAT-1 and AAT-2, resulting in different dimeric isoforms.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-1200976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-14154476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-14907688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-16662397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-16664708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-16665039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-16665469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-3782150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-4193186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-4680825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-5796102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-6162199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-6870268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666973-7294808
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
90
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1622-9
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1989
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| pubmed:articleTitle |
Aspartate aminotransferase in alfalfa root nodules : I. Purification and partial characterization.
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| pubmed:affiliation |
U.S. Department of Agriculture/Agricultural Research Service, University of Minnesota, St. Paul, Minnesota 55108.
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| pubmed:publicationType |
Journal Article
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