Linked Life Data

1666689

Source:http://linkedlifedata.com/resource/pubmed/id/1666689

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-5-5
pubmed:abstractText
We report the construction of a chromosomal malT-lacZ gene fusion that is expressed under the control of the malT promoter in Escherichia coli K12. The resulting hybrid protein is soluble and stable in crude cellular extracts, which allowed us to measure very low levels of malTp activity. In this note, we confirm and extend previous observations on the regulation of malTp. We show that the promoter is 40-times less active in the absence of cAMP receptor protein (CRP) than in its presence, that CRP works by binding to the site centred at position -70.5, and that all of the elements necessary and sufficient for the regulation by CRP are located downstream from position -122.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0923-2508
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
937-42
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Genetic studies on the promoter of malT, the gene that encodes the activator of the Escherichia coli maltose regulon.
pubmed:affiliation
Unité de Génétique moléculaire, Institut Pasteur (URA 1149 du CNRS), Paris.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't