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pubmed-article:16666682pubmed:abstractTextProtoplasts obtained from corn (Zea mays) suspension cells were studied using the whole cell patch-clamp technique. One time-independent current, as well as two time-dependent currents were identified. All three currents were reduced by tetraethylammonium (9 millimolar), a K(+) channel blocker. The time-independent current had a nearly linear current-voltage relationship and its reversal potential, defined as the voltage at which there is zero current, was highly dependent on the extracellular potassium concentration. One of the two time-dependent currents was activated, with rapid kinetics, by membrane hyperpolarization to potentials more negative than -100 millivolts. The second time-dependent current was activated with a sigmoidal time course by membrane depolarization to potentials more positive than -60 millivolts. It exhibited no inactivation and was carried primarily by potassium ions. These characteristics suggest that this latter current is caused by the voltage-dependent opening of delayed-rectifier K(+) channels. These three currents, which are not generated by the plasmalemma H(+)-ATPase, are likely to assist in the regulation of the cellular K(+) fluxes and membrane potential.lld:pubmed
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pubmed-article:16666682pubmed:authorpubmed-author:ShrierAAlld:pubmed
pubmed-article:16666682pubmed:authorpubmed-author:PooleR JRJlld:pubmed
pubmed-article:16666682pubmed:authorpubmed-author:KetchumK AKAlld:pubmed
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pubmed-article:16666682pubmed:volume89lld:pubmed
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pubmed-article:16666682pubmed:pagination1184-92lld:pubmed
pubmed-article:16666682pubmed:dateRevised2010-9-14lld:pubmed
pubmed-article:16666682pubmed:year1989lld:pubmed
pubmed-article:16666682pubmed:articleTitleCharacterization of potassium-dependent currents in protoplasts of corn suspension cells.lld:pubmed
pubmed-article:16666682pubmed:affiliationCentre for Plant Molecular Biology, Department of Biology, McGill University, Montréal, Québec, Canada H3A 1B1.lld:pubmed
pubmed-article:16666682pubmed:publicationTypeJournal Articlelld:pubmed
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