Source:http://linkedlifedata.com/resource/pubmed/id/16666682
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
Protoplasts obtained from corn (Zea mays) suspension cells were studied using the whole cell patch-clamp technique. One time-independent current, as well as two time-dependent currents were identified. All three currents were reduced by tetraethylammonium (9 millimolar), a K(+) channel blocker. The time-independent current had a nearly linear current-voltage relationship and its reversal potential, defined as the voltage at which there is zero current, was highly dependent on the extracellular potassium concentration. One of the two time-dependent currents was activated, with rapid kinetics, by membrane hyperpolarization to potentials more negative than -100 millivolts. The second time-dependent current was activated with a sigmoidal time course by membrane depolarization to potentials more positive than -60 millivolts. It exhibited no inactivation and was carried primarily by potassium ions. These characteristics suggest that this latter current is caused by the voltage-dependent opening of delayed-rectifier K(+) channels. These three currents, which are not generated by the plasmalemma H(+)-ATPase, are likely to assist in the regulation of the cellular K(+) fluxes and membrane potential.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16591089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16593851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16655896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16660164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16661066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16662296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16663928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16664490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-16665712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-17807848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-2415541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-2439078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666682-6492130
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
89
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1184-92
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1989
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| pubmed:articleTitle |
Characterization of potassium-dependent currents in protoplasts of corn suspension cells.
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| pubmed:affiliation |
Centre for Plant Molecular Biology, Department of Biology, McGill University, Montréal, Québec, Canada H3A 1B1.
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| pubmed:publicationType |
Journal Article
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