Linked Life Data

1666651

Source:http://linkedlifedata.com/resource/pubmed/id/1666651

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1992-5-7
pubmed:abstractText
1. The whole-cell patch-clamp technique was used to determine the actions of n-octyl sulphate (OS-) anions and n-octyl trimethylammonium (OTMA+) cations on sodium current steady-state inactivation and peak amplitude in cells isolated from dorsal root ganglia of neonatal rats and maintained in short-term tissue culture. This paper concentrates on the effects of external addition but the actions of internal OS- and OTMA+ are briefly considered. 2. The main action of external OS- was to cause a hyperpolarizing shift in the voltage dependence of the steady-state inactivation parameter, h infinity. At 1-6 mM OS- caused a shift in the mid-point of the h infinity curve of around -30 mV. The shape of the h infinity curve was altered in a concentration-dependent manner. Internal OS- had no discernible effect on the shape or position of the h infinity curve. 3. External OS- produced a relatively small (less than 25%) reduction in the maximum current achieved following pre-pulses sufficiently negative to remove resting steady-state inactivation. 4. By contrast, external OTMA+ had little effect on the voltage dependence of h infinity and produced a small, but significant, increase in the maximum sodium current. 2 mM-external OTMA+ moved the mid-point of the h infinity curve (Vh) 5 mV in the depolarizing direction (relative to the mean of control and reversal curves) and increased the maximum current by 13%. One millimolar internal OTMA+ induced a frequency-dependent current block. 5. Raising the external calcium concentration from 2 to 20 mM (in the presence of 2 mM-magnesium and 5 mM-cobalt) caused an 18 mV depolarizing shift in Vh, consistent with a reduction in the negativity of an external surface charge. The maximum current was reduced by 22%. 6. One millimolar OS- reduced the surface potential of egg phosphatidylcholine (EPC) monolayers (at an air-0.5 M-NaCl interface) by 35 mV but 1 or 2 mM-OTMA+ produced only a 2-3 mV increase. The quantitative agreement between the effects of OS-, on Vh in the rat and on monolayer surface potential, decreased with increasing concentration.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-14946715, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2159518, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2166163, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2166601, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2169040, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2444301, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2550880, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2561786, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2580983, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-2995679, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-304888, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-316452, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-3790564, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-6097678, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-6270629, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-6275294, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-6312030, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-6747855, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-737290, http://linkedlifedata.com/resource/pubmed/commentcorrection/1666651-978495
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:volume
440
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-53
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
The influence of charge on the effects of n-octyl derivatives on sodium current inactivation in rat sensory neurones.
pubmed:affiliation
Department of Anatomy and Physiology, The University, Dundee.
pubmed:publicationType
Journal Article, In Vitro