| pubmed-article:16666170 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16666170 | lifeskim:mentions | umls-concept:C0242726 | lld:lifeskim |
| pubmed-article:16666170 | lifeskim:mentions | umls-concept:C1138842 | lld:lifeskim |
| pubmed-article:16666170 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:16666170 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:16666170 | lifeskim:mentions | umls-concept:C0205390 | lld:lifeskim |
| pubmed-article:16666170 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:16666170 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:16666170 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16666170 | pubmed:dateCreated | 2010-6-29 | lld:pubmed |
| pubmed-article:16666170 | pubmed:abstractText | Crude plasma membranes of corn (Zea mays L.) roots were obtained according to MI De Michelis and RM Spanswick (1986 Plant Physiol 81: 542-547). This preparation, which contained tightly sealed vesicles displaying Mg-ATP dependent H(+)-transport, was purified by phase partitioning. The percentage of inside-out vesicles (10%) was determined from the Mg-ATPase latency, revealed with lysophosphatidylcholine. A Triton X-100 treatment described previously (JP Grouzis, R Gibrat, J Rigaud, C Grignon 1987 Biochim Biophys Acta 903: 449-464) was applied to phase-partitioned plasma membranes. The percentage of catalytic sites freely accessible to Mg-ATP increased to 50% after Triton X-100 treatment. Treated vesicles remained capable of electrogenic H(+)-pumping, as demonstrated by Mg:ATP-dependent quinacrine fluorescence quenching and oxonol absorbance shift. As expected from the large increase of the catalytic sites accessibility, increases of the dye responses were observed. Concanavalin A binding was estimated from microelectrophoretic measurements of individual vesicles. Statistical analysis of concanavalin A binding and Mg-ATPase latency suggest that treated membranes have lost their asymmetric structure. | lld:pubmed |
| pubmed-article:16666170 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666170 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666170 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16666170 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666170 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666170 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666170 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666170 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16666170 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16666170 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:16666170 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16666170 | pubmed:issn | 0032-0889 | lld:pubmed |
| pubmed-article:16666170 | pubmed:author | pubmed-author:RigaudJJ | lld:pubmed |
| pubmed-article:16666170 | pubmed:author | pubmed-author:GrignonCC | lld:pubmed |
| pubmed-article:16666170 | pubmed:author | pubmed-author:BelverAA | lld:pubmed |
| pubmed-article:16666170 | pubmed:author | pubmed-author:GibratRR | lld:pubmed |
| pubmed-article:16666170 | pubmed:author | pubmed-author:GrouzisJ PJP | lld:pubmed |
| pubmed-article:16666170 | pubmed:author | pubmed-author:GaltierNN | lld:pubmed |
| pubmed-article:16666170 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16666170 | pubmed:volume | 87 | lld:pubmed |
| pubmed-article:16666170 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16666170 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16666170 | pubmed:pagination | 491-7 | lld:pubmed |
| pubmed-article:16666170 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
| pubmed-article:16666170 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:16666170 | pubmed:articleTitle | Preparation of Corn Root Plasmalemma with Low Mg-ATPase Latency and High Electrogenic H Pumping Activity after Phase Partitioning. | lld:pubmed |
| pubmed-article:16666170 | pubmed:affiliation | Biochimie et Physiologie Végétales, Institut National de la Recherche Agronomique, 34060 Montpellier Cedex, France. | lld:pubmed |
| pubmed-article:16666170 | pubmed:publicationType | Journal Article | lld:pubmed |
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