Source:http://linkedlifedata.com/resource/pubmed/id/16666170
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
Crude plasma membranes of corn (Zea mays L.) roots were obtained according to MI De Michelis and RM Spanswick (1986 Plant Physiol 81: 542-547). This preparation, which contained tightly sealed vesicles displaying Mg-ATP dependent H(+)-transport, was purified by phase partitioning. The percentage of inside-out vesicles (10%) was determined from the Mg-ATPase latency, revealed with lysophosphatidylcholine. A Triton X-100 treatment described previously (JP Grouzis, R Gibrat, J Rigaud, C Grignon 1987 Biochim Biophys Acta 903: 449-464) was applied to phase-partitioned plasma membranes. The percentage of catalytic sites freely accessible to Mg-ATP increased to 50% after Triton X-100 treatment. Treated vesicles remained capable of electrogenic H(+)-pumping, as demonstrated by Mg:ATP-dependent quinacrine fluorescence quenching and oxonol absorbance shift. As expected from the large increase of the catalytic sites accessibility, increases of the dye responses were observed. Concanavalin A binding was estimated from microelectrophoretic measurements of individual vesicles. Statistical analysis of concanavalin A binding and Mg-ATPase latency suggest that treated membranes have lost their asymmetric structure.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-103582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-13590051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-16662676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-16663096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-16663264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-16664008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-16664853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-263685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-4128882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-6224512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-6290646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666170-7378382
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
87
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
491-7
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1988
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| pubmed:articleTitle |
Preparation of Corn Root Plasmalemma with Low Mg-ATPase Latency and High Electrogenic H Pumping Activity after Phase Partitioning.
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| pubmed:affiliation |
Biochimie et Physiologie Végétales, Institut National de la Recherche Agronomique, 34060 Montpellier Cedex, France.
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| pubmed:publicationType |
Journal Article
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