Linked Life Data

16665823

Source:http://linkedlifedata.com/resource/pubmed/id/16665823

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Sperm cells were isolated from corn (Zea mays L.) tricellular pollen grains. They were released using a light osmotic chock, and separated from pollen contaminants (especially starch grains) by a Percoll gradient centrifugation. Isolated sperm cells (3 x 10(6) per milliliter) show a high viability score (90%) as demonstrated with the fluorochromatic reaction. They appeared as spherical cells which lack cell wall and plastids, and can be considered as haploid protoplasts.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Dec
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
876-8
pubmed:dateRevised
2010-9-15
pubmed:year
1987
pubmed:articleTitle
Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains.
pubmed:affiliation
Université Claude Bernard-Lyon 1, Laboratoire Reconnaissance Cellulaire et Amélioration des Plantes, Bät 741, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France.
pubmed:publicationType
Journal Article