Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-6-29
pubmed:abstractText
The initial step in tumor formation by Agrobacterium tumefaciens is the site-specific attachment of the bacteria to plant cells. A similar attachment to plant tissue culture cells has been observed. Binding to carrot suspension culture cells was not dependent on the presence of divalent cations and was not inhibited by the addition of mannose, alpha-methyl mannoside, galactose, arabinose, glucosamine, 2-deoxyglucose, or 0.25 molar NaCl to the culture medium. The ability of the carrot cells to bind A. tumefaciens was markedly reduced by elution of the cells with dilute detergent or CaCl(2) or by incubation of the cells with proteolytic enzymes. The carrot cells were not killed by these treatments and recovered the ability to bind A. tumefaciens within 3 to 6 hours. A. tumefaciens did not bind to carrot cells which had been induced to form embryos (AG Matthysse, RHG Gurlitz 1982 Physiol Plant Pathol 21: 381-387). A comparison of the peptides eluted from embryos and from uninduced cells using sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that there were several changes in extractable polypeptides after embryo induction. One or more of the polypeptides present before embryo induction and absent from embryos may be involved in the binding of A. tumefaciens to the carrot cell surface.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-16657264, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-16660732, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-16661526, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-16662504, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-16664024, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-336832, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-4556971, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-55100, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-5773014, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-6162199, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-6292165, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-6302086, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-730370, http://linkedlifedata.com/resource/pubmed/commentcorrection/16665289-7462151
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Mar
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
564-8
pubmed:dateRevised
2010-9-14
pubmed:year
1987
pubmed:articleTitle
Involvement of Carrot Cell Surface Proteins in Attachment of Agrobacterium tumefaciens.
pubmed:affiliation
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27514.
pubmed:publicationType
Journal Article