Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-6-29
pubmed:abstractText
1-Aminocyclopropane-1-carboxylic Acid (ACC), the immediate precursor of ethylene is routinely assayed by converting it into ethylene with NaOCl, and the ethylene liberated is then determined by gas chromatography (MCC Lizada, SF Yang 1979 Anal Biochem 100: 140-145). However, certain materials which may be present in crude plant extracts or in enzyme reaction mixtures interfere with this assay procedure. Mono, and di-alkyl amines cause poor yields of ethylene from ACC. Ethanol in the presence of NH(3) or amines but in the absence of ACC can produce ethylene under the assay procedure. The characteristics of these interfering reactions were studied and precautions to avoid these problems are suggested. Recovery of ACC during its extraction and purification from plant extracts were tested and are discussed.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
May
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
156-60
pubmed:dateRevised
2010-9-14
pubmed:year
1986
pubmed:articleTitle
Interferences and specificity of the 1-aminocyclopropane-1-carboxylic Acid assay with the hypochlorite reagent.
pubmed:affiliation
Department of Vegetable Crops, University of California, Davis, California 95616.
pubmed:publicationType
Journal Article