Source:http://linkedlifedata.com/resource/pubmed/id/16664662
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
In previous studies (GL Creason et al. 1983 Biochem Biophys Res Commun 117: 658-662; LP Holowach et al. 1984 Plant Physiol 74: 576-583), we have shown that when soybean (Glycine max L. Merrill cv Provar) cotyledons are cultured in medium supplemented with l-methionine, the beta-subunit of 7S protein and beta-mRNA are absent. We have carried out further studies on the mechanism of the methionine action. In one experiment, cotyledons were cultured for 16 days with or without methionine. After 4 days, some cotyledons were transferred from methionine-supplemented to basal (no methionine) medium and vice versa. In basal medium, beta-subunit was detected at 4 days whereas in methionine-supplemented medium, no beta-subunit was present. When cotyledons were transferred from basal to methionine-supplemented medium, the beta-subunit increased within a 4 day period and then remained constant (on a per cotyledon basis). This result indicated that methionine was not acting by accelerating the degradation of the beta-subunit. Four days after transfer from supplemented to basal medium cotyledons contained beta-subunit, thus demonstrating that the inhibition was reversible. During this time, the uncombined methionine declined from 7 to 1.5 mumoles methionine per gram fresh weight. When beta-mRNA was measured by in vitro translation, functional beta-mRNA was absent in tissue that was not accumulating beta-subunit. The messenger RNA for the beta-subunit had a half-life of about 1 day in the presence of methionine. Hybridization of cotyledon mRNA with cDNA complementary to beta-mRNA revealed that the 1700 nucleotide beta-mRNA was not present in supplemented cotyledons. Thus, expression of the beta-subunit gene is controlled at the level of transcription, RNA processing, or RNA turnover, rather than at the level of translation.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-1175627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-16660248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-16663463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-16663464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-16663567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-16663681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-16748534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-17746179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-4311742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-556958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-6113180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-6287227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-6356981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-6428392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-6686764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-6897678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-7119014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-7202034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-856800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664662-942997
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
80
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
561-7
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1986
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| pubmed:articleTitle |
Studies on the Mechanism of Regulation of the mRNA Level for a Soybean Storage Protein Subunit by Exogenous l-Methionine.
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| pubmed:affiliation |
United States Plant, Soil and Nutrition Laboratory, Agricultural Research Service, United States Department of Agriculture, Ithaca, New York 14853.
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| pubmed:publicationType |
Journal Article
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