Source:http://linkedlifedata.com/resource/pubmed/id/16664408
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
A method for the induction of a high rate of cell division and embryogenesis of Nicotiana rustica pollen was developed. Binucleate pollen grains were fractionated by Percoll density gradient (35/45%) centrifugation and cultured in 0.4 molar mannitol at 30 degrees C (the first culture). After 3 days in culture pollen was recollected by a second Percoll fractionation (0/30%) and transferred to and cultured in a medium containing the Murashige-Skoog macro-elements, 0.4 molar mannitol, 40 millimolar galactose, 3 millimolar glutamine, and 5 micromolar ABA for 10 days (the second culture). The cell population consisting of about 80% dividing pollen was transferred to a Murashige-Skoog medium containing 0.4 molar mannitol, 3 millimolar glutamine, and no phytohormone (the third culture), where about 40% of dividing pollen developed into embryos or embryogenic calli.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
79
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
90-4
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| pubmed:year |
1985
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| pubmed:articleTitle |
Studies on Conditions for Cell Division and Embryogenesis in Isolated Pollen Culture of Nicotiana rustica.
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| pubmed:affiliation |
Institute of Biological Sciences, The University of Tsukuba, Sakura-mura, Ibaraki 305, Japan.
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| pubmed:publicationType |
Journal Article
|