Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Sealed membrane vesicles were isolated from homogenates of sugarbeet (Beta vulgaris L.) taproot by a combination of differential centrifugation, extraction with KI, and dextran gradient centrifugation. Relative to the KI-extracted microsomes, the content of plasma membranes, mitochondrial membranes, and Golgi membranes was much reduced in the final vesicle fraction. A component of ATPase activity that was inhibited by nitrate co-enriched with the capacity of the vesicles to form a steady state pH gradient during the purification procedure. This suggests that the nitrate-sensitive ATPase may be involved in driving H(+)-transport, and this is consistent with the observation that H(+)-transport, in the final vesicle fraction was inhibited by nitrate. Proton transport in the sugarbeet vesicles was substrate specific for ATP, insensitive to sodium vanadate and oligomycin but was inhibited by diethylstilbestrol and N,N'-dicyclohexylcarbodiimide. The formation of a pH gradient in the vesicles was enhanced by halide ions in the sequence I(-) > Br(-) > Cl(-) while F(-) was inhibitory. These stimulatory effects occur from both a direct stimulation of the ATPase by anions and a reduction in the vesicle membrane potential. In the presence of Cl(-), alkali cations reduce the pH gradient relative to that observed with bis-tris-propane, possibly by H(+)/alkali cation exchange. Based upon the properties of the H(+)-transporting vesicles, it is proposed that they are most likely derived from the tonoplast so that this vesicle preparation would represent a convenient system for studying the mechanism of transport at this membrane boundary.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-137147, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16592894, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16662676, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16662754, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16662829, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16662875, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16663096, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16663457, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16663459, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16664026, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-16664343, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-383007, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-4885777, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-6250453, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-6339489, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-6358501, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-6358502, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-7253009, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664342-942051
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Aug
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
865-70
pubmed:dateRevised
2010-9-14
pubmed:year
1985
pubmed:articleTitle
Membrane transport in isolated vesicles from sugarbeet taproot : I. Isolation and characterization of energy-dependent, h-transporting vesicles.
pubmed:affiliation
Plant Biochemistry and Bioregulation Laboratory, United States Department of Agriculture, Agricultural Research Service, Utah State University, Logan, Utah 84322.
pubmed:publicationType
Journal Article