Linked Life Data

16664324

Source:http://linkedlifedata.com/resource/pubmed/id/16664324

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Nitrogen metabolism was examined in senescent flag leaves of 90- to 93-day-old wheat (Triticum aestivum L. cv Yecora 70) plants. CO(2) assimilation and the levels of protein, chlorophyll, and nitrogen in the leaves decreased with age. Glutamine synthetase activity decreased to one-eighth of the level in young flag leaves. Detached leaves were incubated (with the cut base) in (15)N-labeled NH(3), glutamate, or glycine in the light (1.8 millieinstein per square meter per second) at 25 degrees C in an open gas exchange system under normal atmospheric conditions for up to 135 minutes. The (15)N-enrichment of various amino acids derived from these (15)N-substrates were examined. The amido-N of glutamine was the first (15)N-labeled product in leaves incubated with (15)NH(4)Cl whereas serine, closely followed by the amido- and amino-N of glutamine, were the most highly (15)N-labeled products during incubation with [(15)N]glycine. In contrast, aspartate and alanine were the first (15)N-labeled products when [(15)N] glutamate was used. These results indicate that NH(3) was assimilated via glutamine synthetase and glutamate synthase activities and the photorespiratory nitrogen cycle remained functional in these senescent wheat flag leaves. In contrast, an involvement of glutamate dehydrogenase in the assimilation of ammonia could not be detected in these tissues.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Aug
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
779-83
pubmed:dateRevised
2010-9-14
pubmed:year
1985
pubmed:articleTitle
Nitrogen Metabolism in Senescent Flag Leaves of Wheat (Triticum aestivum L.) in the Light.
pubmed:affiliation
Fachbereich Biologie der Universitaet Kaiserslautern, Postfach 3049, 6750 Kaiserslautern, Germany.
pubmed:publicationType
Journal Article