Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Endoproteinase activities and species were compared during dark-induced senescence of attached and detached primary barley leaves by isoelectric focusing and polyacrylamide gel electrophoresis of cell-free extracts. Neither of the two major endoproteinases (EP1 and EP2) changed in amounts during senescence of attached leaves, nor did new endoproteinases appear. In contrast, during senescence of detached leaves, both EP1 and EP2 activities increased and four new species of endoproteinases appeared. Proteolytic activity was evenly distributed throughout attached leaves, but activity in the detached leaf increased sharply from the tip to the base with the four new higher molecular weight species of proteinases present only in the bottom half of the leaf nearest the cut end. Thus a wound response may be superimposed on the processes of senescence in detached leaves. Cycloheximide and kinetin both inhibited the increase of EP1, EP2, and the induction of the four new endoproteinases; chloramphenicol had no effect. Indications are that both the increases in activity and the induction of new species of proteinases were the result of activity of cytoplasmic ribosomes.Hydrolysis of total protein and ribulose-1,5-bisphosphate carboxylase protein in vivo was somewhat faster in detached than attached leaves. The difference, however, was much less than would be expected from the great increase in proteolytic activity in detached leaves.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-14240533, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-14240539, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16654194, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16657477, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16657898, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16659200, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16659836, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16660567, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16660869, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16661069, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16661340, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16661606, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16661803, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16662028, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16662055, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16662184, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16662193, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16662252, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-16663604, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/16664263-5479291
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jul
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
442-6
pubmed:dateRevised
2010-9-14
pubmed:year
1985
pubmed:articleTitle
Differential Induction of Endoproteinases during Senescence of Attached and Detached Barley Leaves.
pubmed:affiliation
Plant Growth Laboratory and the Department of Agronomy and Range Science, University of California, Davis, California 95616.
pubmed:publicationType
Journal Article