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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1992-4-28
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pubmed:abstractText |
Tiamulin with an IC50 of 1.7 x 10(-6) M inhibited both the rapid and sustained contractions induced by hyperosmotically added 60 mM K+ (Hyper 60 K+) without changing the membrane potential in the intestinal muscle. Tiamulin inhibition (2 x 10(-6)-2 x 10(-5) M) of the Ca(2+)-induced contraction in depolarized muscle was competitively antagonized by raising external Ca2+. Tiamulin (2 x 10(-5) M) slightly affected the Hyper 60 K(+)-induced phasic contraction under hypoxia and the carbachol-induced phasic contraction. Moreover, tiamulin (2 x 10(-5) M) inhibited the Hyper 60 K(+)-induced contraction with decreasing [Ca2+]cyt level. Although the inhibitory effect of 10(-7)-10(-5) M monesin, an inhibitor of mitochondrial respiration, on the Hyper 60 K(+)-induced contraction was reduced under hypoxia, the effect of tiamulin (2 x 10(-7)-2 x 10(-4) M) was not modified. Tiamulin changed neither the intracellular Na+ and K+ content of the depolarized muscle nor the Ca(2+)-induced contraction in the chemically skinned preparations. These results suggest that the inhibitory action of tiamulin on the Hyper 60 K(+)-induced tonic contraction is possibly due to the competitive inhibition of Ca2+ entry through the voltage-dependent Ca2+ channel of the intestinal smooth muscle cell.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil,
http://linkedlifedata.com/resource/pubmed/chemical/tiamulin
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-5198
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
591-600
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1666422-Animals,
pubmed-meshheading:1666422-Anti-Bacterial Agents,
pubmed-meshheading:1666422-Calcium,
pubmed-meshheading:1666422-Calcium Channels,
pubmed-meshheading:1666422-Diterpenes,
pubmed-meshheading:1666422-Electrophysiology,
pubmed-meshheading:1666422-Guinea Pigs,
pubmed-meshheading:1666422-Intestines,
pubmed-meshheading:1666422-Male,
pubmed-meshheading:1666422-Membrane Potentials,
pubmed-meshheading:1666422-Muscle, Smooth,
pubmed-meshheading:1666422-Muscle Contraction,
pubmed-meshheading:1666422-Potassium,
pubmed-meshheading:1666422-Verapamil
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pubmed:year |
1991
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pubmed:articleTitle |
The inhibitory effect of tiamulin on high K(+)-induced contraction in guinea pig intestinal smooth muscle.
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pubmed:affiliation |
Division of Veterinary Pharmacology, Nippon Veterinary and Animal Science University, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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