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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
An anion-sensitive ATP-dependent H(+) transport in microsomal membranes from Zea mays L. coleoptiles was partially characterized using the pH gradient-dependent decrease of unprotonated neutral red. The following criteria strongly suggest a tonoplast origin of the H(+) transport observed: strict dependence on Cl(-); inhibition by SO(4) (2-) and NO(3) (-); insensitivity against vanadate, molybdate, and azide; reversible inhibition by CaCl(2) (H(+)/Ca(2+) antiport); inhibition by diethylstilbestrol. The substrate kinetics revealed simple Michaelis Menten kinetics for ATP in the presence of 1 millimolar MgCl(2) with a K(m) value of 0.56 millimolar (0.38 millimolar for MgATP). AMP and c-AMP did not influence H(+) transport significantly. However, ADP was a potent competitive inhibitor with a K(i) value of 0.18 millimolar. The same inhibition type was found for membranes prepared from primary roots by the same procedure.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16662875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16663096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16663457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16663458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-16663459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-4238439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16664155-6255697
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
881-5
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pubmed:dateRevised |
2010-9-14
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pubmed:year |
1985
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pubmed:articleTitle |
ADP Is a Competitive Inhibitor of ATP-Dependent H Transport in Microsomal Membranes from Zea mays L. Coleoptiles.
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pubmed:affiliation |
Botanisches Institut der J. W. Goethe-Universität, Siesmayerstrasse 70, 6000-Frankfurt, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article
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