Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-6-29
pubmed:abstractText
The effect of vanadate on the plant plasma membrane ATPase were investigated in plasma membrane fractions derived from corn roots (Zea mays L.) and red beets (Beta vulgaris L.). The K(i) for vanadate inhibition of the plasma membrane ATPase from corn roots and red beets was between 6 and 15 micromolar vanadate. In both membrane fractions, 80% to 90% of the total ATPase was inhibited at vanadate concentrations below 100 micromolar. Vanadate inhibition was optimal at pH 6.5, enhanced by the presence of K(+), and was partially reversed by 1 millimolar EDTA. The Mg:ATP kinetics for the plasma membrane ATPase were hyperbolic in both the absence and presence of vanadate. Vanadate decreased both the K(m) and V(max) of the red beet plasma membrane ATPase, indicating that vanadate inhibits the ATPase uncompetitively. These results indicate many similarities with respect to vanadate inhibition between the plant plasma membrane ATPase and other major iontranslocating ATPases from fungal and animal cells. The high sensitivity to vanadate reported here, however, differs from other reports of vanadate inhibition of the plant plasma membrane ATPase from corn, beets, and in some instances oats.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-144127, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-155060, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-155066, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-158359, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16593251, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16661634, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16661950, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16662523, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16662676, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16662857, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16662938, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16663096, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-16663457, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-212422, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-4128882, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-6210692, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-6215404, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-6446933, http://linkedlifedata.com/resource/pubmed/commentcorrection/16663670-6457834
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jul
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
586-91
pubmed:dateRevised
2010-9-14
pubmed:year
1984
pubmed:articleTitle
Effects of vanadate on the plasma membrane ATPase of red beet and corn.
pubmed:affiliation
Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
pubmed:publicationType
Journal Article