Source:http://linkedlifedata.com/resource/pubmed/id/16663547
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
Partial digestion of polygalacturonic acid with polygalacturonase isolated from Rhizopus stolonifer produces a mixture of alpha-1,4-d-galacturonide oligomers which act to elicit casbene synthetase activity in castor bean (Ricinus communis L). These oligomers were separated by anion exchange chromatography on DEAE Sephadex A-25 into discrete sizes and their degrees of polymerization were analyzed by fast atom bombardment mass spectrometry. A minimum degree of polymerization of nine units appears to be required for elicitor activity; trideca-alpha-1,4-d-galacturonide was the most active of the oligomers tested. Methyl-esterification of the carboxylate groups greatly diminishes the elicitor activity of the oligomers, a finding which suggests a requirement for the polyanionic character of the oligomers for full activity. The fact that a number of other polyanionic polymers tested as casbene synthetase elicitors did not show significant activity indicates that structural features other than the polyanionic character are also necessary for activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663547-16661728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663547-16662068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663547-16662367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663547-16662929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663547-4269305
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pubmed:language |
eng
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pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0032-0889
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
989-92
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pubmed:dateRevised |
2010-9-14
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pubmed:year |
1984
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pubmed:articleTitle |
Characteristics of galacturonic Acid oligomers as elicitors of casbene synthetase activity in castor bean seedlings.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, California 90024.
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pubmed:publicationType |
Journal Article
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