Source:http://linkedlifedata.com/resource/pubmed/id/16663042
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
The biochemical basis for the ability of the pterocarpan phytoalexin glycinol (3,6a,9-trihydroxypterocarpan) to inhibit the growth of bacteria was examined. Glycinol at bacteriostatic concentrations (e.g. 50 micrograms per milliliter) inhibits the ability of Erwinia carotovora to incorporate [(3)H]leucine, [(3)H]thymidine, or [(3)H]uridine into biopolymers. Exposure of Escherichia coli membrane vesicles to glycinol at 20 micrograms per milliliter results in inhibition of respiration-linked transport of [(14)C]lactose and [(14)C]glycine into the vesicles when either d-lactate or succinate is supplied as the energy source. The ability of E. coli membrane vesicles to transport [(14)C]alpha-methyl glucoside, a vectorial phosphorylation-mediated process, is also inhibited by glycinol at 20 micrograms per milliliter. Furthermore, exposure of membrane vesicles to glycinol (50 micrograms per milliliter) at 20 degrees C results in the leakage of accumulated [(14)C]alpha-methyl glucoside-6-phosphate. The effects of the phytoalexins glyceollin, capsidiol, and coumestrol, and daidzein, a compound structurally related to glycinol but without antibiotic activity, upon the E. coli membrane vesicle respiration-linked transport of [(14)C]glycine and of [(14)C]alpha-methyl glucoside was also examined. Glyceollin and coumestrol (50 micrograms per milliliter), but not daidzein, inhibit both membrane-associated transport processes. These data imply that the antimicrobial activity of glycinol, glyceollin, and coumestrol are due to a general interaction with the bacterial membrane. Capsidiol (50 micrograms per milliliter) inhibits d-lactate-dependent transport of [(14)C]glycine but not vectorial phosphorylation-mediated transport of [(14)C]alpha-methyl glucoside. Thus, capsidiol's mechanism of antimicrobial action seems to differ from that of the other phytoalexins examined.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-13395009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-14070393,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-197881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-359568,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-4316677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-4331061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-4344983,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-4869559,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16663042-942051
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
72
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
557-63
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1983
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| pubmed:articleTitle |
Host-Pathogen Interactions : XXIII. The Mechanism of the Antibacterial Action of Glycinol, a Pterocarpan Phytoalexin Synthesized by Soybeans.
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| pubmed:affiliation |
Department of Chemistry, University of Colorado, Boulder, Colorado 80309.
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| pubmed:publicationType |
Journal Article
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