Source:http://linkedlifedata.com/resource/pubmed/id/16662774
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2010-6-29
|
| pubmed:abstractText |
Nitrate reductase-deficient barley (Hordeum vulgare L.) mutants were assayed for the presence of a functional molybdenum cofactor determined from the activity of the molybdoenzyme, xanthine dehydrogenase, and for nitrate reductase-associated activities. Rocket immunoelectrophoresis was used to detect nitrate reductase cross-reacting material in the mutants. The cross-reacting material levels of the mutants ranged from 8 to 136% of the wild type and were correlated with their nitrate reductase-associated activities, except for nar 1c, which lacked all associated nitrate reductase activities but had 38% of the wild-type cross-reacting material. The cross-reacting material of two nar 1 mutants, as well as nar 2a, Xno 18, Xno 19, and Xno 29, exhibited rocket immunoprecipitates that were similar to the wild-type enzyme indicating structural homology between the mutant and wild-type nitrate reductase proteins. The cross-reacting materials of the seven remaining nar 1 alleles formed rockets only in the presence of purified wild-type nitrate reductase, suggesting structural modifications of the mutant cross-reacting materials. All nar 1 alleles and Xno 29 had xanthine dehydrogenase activity indicating the presence of functional molybdenum cofactors. These results suggest that nar 1 is the structural gene for nitrate reductase. Mutants nar 2a, Xno 18, and Xno 19 lacked xanthine dehydrogenase activity and are considered to be molybdenum cofactor deficient mutants. Cross-reacting material was not detected in uninduced wild-type or mutant extracts, suggesting that nitrate reductase is synthesized de novo in response to nitrate.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-14085568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-157059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-16661311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-16662370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-389305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-40454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-4321854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-6460156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662774-770463
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0032-0889
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
71
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
145-9
|
| pubmed:dateRevised |
2010-9-15
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Nitrate reductase-deficient mutants in barley : immunoelectrophoretic characterization.
|
| pubmed:affiliation |
Department of Agronomy and Soils and Program in Genetics and Cell Biology, Washington State University, Pullman, Washington 99164-6420.
|
| pubmed:publicationType |
Journal Article
|