Source:http://linkedlifedata.com/resource/pubmed/id/16662563
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
Immobilization of lettuce (Lactuca sativa) thylakoids has been performed by using glutaraldehyde and bovine serum albumin. Confirming previous reports, a stabilization of the O(2) evolution activity of the photosystem II (PSII) under storage and functional conditions has been observed. The present work is devoted to the role played by mono-and divalent cations, during the immobilization process itself, on the O(2) production. Four types of measurements have been employed: kinetic measurements, low temperature (77 K) fluorescence emission, photoacoustic (PA) spectroscopy, and electron microscopy observations. We show that the effect of glutaraldehyde is complex because it acts as an inhibitor, a stabilizing agent, and a cross-linking reactive. In the present studies, the thylakoids are immobilized within a polymeric insoluble albumin matrix. The highest activity yield and the best storage conditions are obtained when 0.15 mm Na(+) (or K(+)), 1 mm Mg(2+), and 0.1 mm Mn(2+) are present in the resuspending media before the immobilization. Due to modifications of the ionic content during such a process, structural differences are observed on the stacking degree of thylakoids. No modification of the fluorescence and PA spectra after the immobilization are found. Furthermore, a correlation between activities and spectral changes have been shown: when the activities increase, the F(735) to F(695) ratio increases and the PA(676) to PA(440) ratio decreases.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-112922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-1269762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-16656286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-16659564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-16661673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-36298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-4149767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-4460876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-5416111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-5689013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-680141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-6994590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-7002985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-7396509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-7400870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-7417417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-823976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16662563-952523
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
70
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
714-22
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1982
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| pubmed:articleTitle |
Immobilized thylakoids in a cross-linked albumin matrix: effects of cations studied by electron microscopy, fluorescence emission, photoacoustic spectroscopy, and kinetic measurements.
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| pubmed:affiliation |
Laboratoire de Technologie Enzymatique (ERA No. 338 CNRS), Université de Technologie de Compiègne, B.P. 233-60206 Compiègne, France.
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| pubmed:publicationType |
Journal Article
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