Linked Life Data

16661467

Source:http://linkedlifedata.com/resource/pubmed/id/16661467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Polysomes isolated from a susceptible variety of wheat leaves (cultivar W2691) and those inoculated with the wheat stem rust fungus (f. sp. tritici, race 126-ANZ-6, 7) were incubated in a cell-free protein-synthesizing system. Under these conditions, different size classes of polypeptides, ranging in molecular weight from 10,000 to 80,000, are radiolabeled. Using double-isotope labeling technique, we show that some discrete size classes of polypeptides are synthesized in significantly greater quantitites by polysomes from inoculated leaves compared to the corresponding size classes synthesized by polysomes from healthy leaves. These results confirm our previous observation that there are significant changes in the wheat leaf polysomal messenger RNA populations at 3 days after inoculation with the rust fungus.The effects of the organelle-specific inhibitors of protein synthesis, chloramphenicol and lincomycin, on in vitro polysomal messenger RNA translation were investigated. The polypeptides synthesized by polysomes from healthy and inoculated leaves in the presence of chloramphenicol were compared. The results show that, even in the presence of this antibiotic, the polysomes from inoculated leaves synthesize greater quantities of some size classes of polypeptides. These data indicate that changes in polysomal messenger RNA populations involve, at least in part, cytoplasmic messenger RNA.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-1018319, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-1262350, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-270712, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-4206201, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-4212463, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-4748357, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-4818560, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-4857855, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-5110397, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-5126907, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-5246002, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-5416539, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-5667290, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-5762399, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-5803285, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-6028047, http://linkedlifedata.com/resource/pubmed/commentcorrection/16661467-643615
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Sep
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
520-4
pubmed:dateRevised
2010-9-14
pubmed:year
1980
pubmed:articleTitle
Changes in Wheat Leaf Polysomal Messenger RNA Populations during the Early Stages of Rust Infection: EFFECTS OF CHLORAMPHENICOL AND LINCOMYCIN ON CELL-FREE TRANSLATION BY POLYSOMES FROM HEALTHY AND INFECTED LEAVES.
pubmed:affiliation
Laboratory of Plant Biochemistry and Molecular Pathology, Department of Biochemistry, University of Queensland, St. Lucia, Queensland 4067 Australia.
pubmed:publicationType
Journal Article