Linked Life Data

16660860

Source:http://linkedlifedata.com/resource/pubmed/id/16660860

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Amino compounds representative of the major N solutes of xylem sap were pulse-fed (10 to 20 minutes) singly in (14)C-labeled form to cut transpiring shoots of white lupin (Lupinus albus L.). (14)C distribution was studied by autoradiography and radioassays of phloem sap, leaflet tissues, and shoot parts harvested at intervals after labeling. Primary distribution of N by xylem was simulated using a 20-minute labeling pulse followed by a 30-minute chase in unlabeled xylem sap. Shoots fed (14)C-labeled asparagine, glutamine, valine, serine, or arginine showed intense labeling of leaflet veins and marked retention (35 to 78%) of (14)C by stem + petioles. Shoots fed (14)C-labeled aspartic acid or glutamic acid showed heaviest (14)C accumulation in interveinal regions of leaflets and low uptake (11 to 20%) of (14)C by stem + petioles. Departing leaf traces were major sites of uptake of all amino compounds, and the implications of this were evaluated. Fruits acquired only 1 to 5% of the fed label directly from xylem, but more than doubled their intake during the period 30 to 160 minutes after feeding through receipt of (14)C transferred from xylem to phloem in stem and leaves. (14)C-Labeled asparagine and valine transferred directly from xylem to phloem, but the (14)C of (14)C-labeled aspartic acid and arginine appeared in phloem mainly as metabolic products of the fed compound. The labeling of the soluble pool of leaflets reflected these differences. The significance of heterogeneity in distribution and metabolism of xylem amino compounds in the shoot was discussed.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jun
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1076-81
pubmed:dateRevised
2010-9-15
pubmed:year
1979
pubmed:articleTitle
Uptake and Utilization of Xylem-borne Amino Compounds by Shoot Organs of a Legume.
pubmed:affiliation
Department of Botany, University of Western Australia, Nedlands, Western Australia 6009.
pubmed:publicationType
Journal Article