|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2010-6-29
|
|
pubmed:abstractText |
A uracil phosphoribosyltransferase (UMP-pyrophosphorylase) was found in several angiosperms and was partially purified from epicotyls of pea (Pisum sativum L. cv. Alaska) seedlings. Its pH optimum was about 8.5; its required approximately 0.3 mm MgCl(2) for maximum activity but was inhibited by MnCl(2); its molecular weight determined by chromatography on Sephadex G-150 columns was approximately 100,000; its K(m) values for uracil and 5-phosphorylribose 1-pyrophosphate were 0.7 mum and 11 mum; and it was partially resolved from a similar phosphoribosyltransferase converting orotic acid to orotodine 5'-phosphate. Enzyme fractions containing both uracil phosphoribosyl transferase and orotate phosphoribosyltransferase converted 6-azauracil and 5-fluorouracil to products with chromatographic properties of 6-azauradine 5'-phosphate and 5-fluorouridine 5'-phosphate. Uracil phosphoribosyltransferase probably functions in salvage of uracil for synthesis of pyrimidine nucleotides.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-1171096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-13438895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-13449076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-13804595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-13813703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-14325973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-16657849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-4306640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-4355548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-4361640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-4674424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-5063073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-5781408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-5831105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-5862401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-6040896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-6049227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660311-6052429
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:status |
PubMed-not-MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0032-0889
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
61
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
442-6
|
|
pubmed:dateRevised |
2010-9-14
|
|
pubmed:year |
1978
|
|
pubmed:articleTitle |
Properties of pea seedling uracil phosphoribosyltransferase and its distribution in other plants.
|
|
pubmed:affiliation |
Department of Botany and Plant Pathology, Colorado State University, Fort Collins, Colorado 80523.
|
|
pubmed:publicationType |
Journal Article
|
