Source:http://linkedlifedata.com/resource/pubmed/id/16660058
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
Kinetin, N(6)-furfuryladenine, was incorporated into tobacco (nicotiana tabacum L., var. Wis. No. 38) callus RNA isolated from rapidly growing tissue cultured in the presence of N(6)-furfuryladenine-8-(14)C or unlabeled kinetin. Approximately 0.7% of the radioactivity in the labeled kinetin added to the medium was recovered as N(6)-furfuryladenosine (fr(6)A) in the rRNA and tRNA preparations from the tobacco callus. The rRNA contained over 90% of these fr(6) A moieties. The extent of kinetin incorporation was four times greater than that observed for N(6)-benzyladenine. The radiochemical purity of the recovered fr(6) A was confirmed by three successive chromatographic purifications on Sephadex columns (LH-20 eluted with 35% ethanol, G-10 eluted with 20% ethanol, and LH-20 eluted with water). A cytokinin-active ribonucleoside with elution volumes corresponding to fr(6) A was isolated from the tobacco callus rRNA preparation. This compound was analyzed by gas-liquid chromatography and rigorously characterized as N(6)-furfuryladenosine by gas-liquid chromatography-mass spectrometry of the trimethylsilyl derivative.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-14211613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-16657337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-16657339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-16658962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-16659180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-16659417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-4371181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-4557059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-4896715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-4910306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-4927344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-5114982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-5169930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-5257141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16660058-5656249
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
60
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
197-202
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1977
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| pubmed:articleTitle |
Kinetin incorporated into tobacco callus ribosomal RNA and transfer RNA preparations.
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| pubmed:affiliation |
Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wisconsin 53706.
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| pubmed:publicationType |
Journal Article
|