Source:http://linkedlifedata.com/resource/pubmed/id/16659919
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2010-6-29
|
| pubmed:abstractText |
Experiments were undertaken to identify and characterize control mechanisms in tobacco leaf tissue which decrease the relative contribution of photorespiratory CO(2) release and thereby increase net photosynthetic CO(2) fixation. A number of metabolites were supplied to illuminated leaf discs and their effect on the inhibition of glycolate synthesis was measured. Glycolate accumulation, in the presence of alpha-hydroxy-2-pyridinemethanesulfonic acid, was inhibited in leaf discs previously floated on 30 mM solutions of either L-glutamate, L-aspartate, phospho-enolpyruvate, or glyoxylate. The effect of glutamate on glycolate synthesis, which was investigated in detail, was concentration- and time-dependent. Glycolate synthesis was inhibited about 40% by treating leaf discs with 30 mM glutamate, and the inhibition continued for more than 4 hours after the glutamate solution was removed.The glutamate inhibition of glycolate synthesis was accompanied by a marked decrease in the rate of photorespiratory CO(2) release and by maximal increases of about 25% in net photosynthetic CO(2) fixation. The products of (14)CO(2) fixation in leaf discs previously treated with glutamate showed a decrease in glycine (26%), serine (12%), and the stronger acids (18%), and an increase in the neutral compounds (26%) in comparison with discs floated only on water.Data are presented which question whether a catabolite of glutamate or the amino acid itself is responsible for the results observed. These experiments support the view that a genetic selection strategy based on the metabolic control of photorespiration would result in large increases in net photosynthetic CO(2) assimilation in species with high rates of photorespiration.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-1094907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-16654227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-16656976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-16656977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-16657413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-16657791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-16658494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-16659387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-17740019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-4331471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-4370561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659919-5144745
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0032-0889
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
59
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
688-94
|
| pubmed:dateRevised |
2010-9-14
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
Metabolic regulation of glycolate synthesis, photorespiration, and net photosynthesis in tobacco by L-glutamate.
|
| pubmed:affiliation |
Department of Biochemistry, The Connecticut Agricultural Experiment Station, NW Haven, Connecticut 06504.
|
| pubmed:publicationType |
Journal Article
|