Source:http://linkedlifedata.com/resource/pubmed/id/16659004
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
A systematic evaluation disclosed the following conditions to be optimum for the isolation of viable protoplasts from cultured cells of Nicotiana tabacum L. ;Bright Yellow' grown in liquid suspensions: (a) the cell culture in the early phase of cell number increase, (b) an enzyme mixture of 1% cellulase "Onozuka" and 0.2% Macerozyme, (c) an enzyme solution pH of 4.7 or 5.7, (d) a 2- to 3-hr incubation period, (e) 5 ml of enzyme solution per 500 mg cells and contained in a 50-ml Delong flask, (f) agitation on a gyrotory shaker at 50 rpm, and (g) 0.3 to 0.8 m mannitol as osmoticum in the cell enzyme mixture. The incubation temperature may be varied from 22 to 37 C. The procedure enabled 30% of the tobacco cells to form protoplasts, 80% of which regenerated cell walls in 4 days and 40% resumed cell division activity when returned to cell culture medium.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659004-16056900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659004-16592009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659004-16657433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659004-5216367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16659004-5531376
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pubmed:language |
eng
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pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0032-0889
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
936-44
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pubmed:dateRevised |
2010-9-15
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pubmed:year |
1974
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pubmed:articleTitle |
Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells.
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pubmed:affiliation |
Department of Plant Sciences, University of California, Riverside, California 92502.
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pubmed:publicationType |
Journal Article
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