Source:http://linkedlifedata.com/resource/pubmed/id/16658822
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
Ammonium sulfate chromatography has been employed to separate glyceraldehyde 3-phosphate dehydrogenases (GPD) of Sinapis alba cotyledons of various developmental stages. Cotyledons of dark-grown seedlings possess one major NAD-specific enzyme designated NAD-GPD I. Irradiation with continuous far red light leads to a strong increase in NADP-GPD activity and to the formation of a second NAD activity designated NAD-GPD II. These two activities occur in a constant ratio during cotyledon development, and they are eluted together in ammonium sulfate chromatography. In a later stage of cotyledon development the light-dependent increase in NAD-GPD II is matched by an equivalent decrease in NAD-GPD I. These data suggest that the chloroplast marker enzyme NADP-GPD (EC 1.2.1.13) also has NAD activity and that the light-dependent formation of this bifunctional enzyme is correlated with activity changes of the NAD-GPD of cytoplasmic glycolysis (EC 1.2.1.12).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-13271335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-14192894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-16655796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-16656206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-16658301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-4386919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-4387009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-4389123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-4390756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-4395130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16658822-4396069
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pubmed:language |
eng
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pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0032-0889
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
100-4
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pubmed:dateRevised |
2010-9-15
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pubmed:year |
1974
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pubmed:articleTitle |
Glyceraldehyde 3-Phosphate Dehydrogenases and Glyoxylate Reductase: II. Far Red Light-Dependent Development of Glyceraldehyde 3-Phosphate Dehydrogenase Isozyme Activities in Sinapis Alba Cotyledons.
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pubmed:affiliation |
Biologisches Institut II, Universität Freiburg im Breisgau, 78 Freiburg, Schänzlestrasse 9-11, Germany.
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pubmed:publicationType |
Journal Article
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