Linked Life Data

1665530

Source:http://linkedlifedata.com/resource/pubmed/id/1665530

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-4-8
pubmed:abstractText
New conditions for quantitative autoradiographic determination of natriuretic peptide (ANP) binding in rat brain were developed in order to inhibit hormone degradation during incubation without the use of proteinase inhibitors. This procedure includes 1-h preincubation of tissue slices in hypotonic buffer and incubation at 4 degrees C in the presence of 4% proteinase-free albumin. ANP binding to choroid plexus and subfornical organ under above conditions reached equilibrium after 90 min. ANP degradation at the end of the incubation did not exceed 2%. Binding data analysis revealed the previously undetected presence of two distinct ANP binding sites in the rat choroid plexus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
A modified quantitative autoradiographic assay for atrial natriuretic peptide receptors in rat brain.
pubmed:affiliation
Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article, In Vitro