Linked Life Data

16647722

Source:http://linkedlifedata.com/resource/pubmed/id/16647722

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2006-8-14
pubmed:abstractText
Regulation of cellular Ca(2+) cycling is central to myocardial contractile function. Loss of Ca(2+) regulation is associated with cardiac dysfunction and pathology. Estrogen has been shown to modify contractile function and to confer cardioprotection. Therefore, we investigated the effect of estrogen on expression of rat heart myocardial Ca(2+)-handling proteins and beta-adrenergic receptor (beta(1)-AR) and examined functional correlates. Female rats were sham-operated (SHAM) or ovariectomized. Two weeks after ovariectomy rats were injected (i.p.) daily with estradiol benozoate (OVX+EB) or sesame oil (OVX) for 2 weeks. Protein abundance was measured by immunoblotting and mRNA was quantified by real-time RT-PCR. OVX significantly decreased estrogen and progesterone levels and EB replacement returned both estrogen and progesterone to physiological levels. OVX induced a 75% reduction of uterine weight and a gain in body weight. Replacement restored weights to SHAM level. OVX increased and estrogen-replacement normalized abundance of beta(1)-AR and L-type Ca(2+) channel (Cav1.2) protein. OVX decreased sodium-Ca(2+) exchange protein (NCX) and estrogen restored protein abundance to SHAM levels. Sarcoplasmic reticular ATPase (SERCA), phospholamban (PLB), and ryanodine receptor (RyR) abundance was not altered by hormone status. Levels of mRNA encoding for beta(1)-AR, Cav1.2, and NCX were not influenced by OVX or estrogen replacement. OVX had no effect on SERCA and PLB mRNA level but estrogen replacement elicited a significant increase compared to OVX and SHAM. Estrogen-dependent changes in Ca(2+)-handling proteins and beta(1)-AR are theoretically consistent reduced myocellular Ca(2+) load. However, hormone-dependent alterations in protein were not associated with changes in contractile function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1257-67
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16647722-Animals, pubmed-meshheading:16647722-Calcium, pubmed-meshheading:16647722-Calcium Channels, L-Type, pubmed-meshheading:16647722-Calcium-Binding Proteins, pubmed-meshheading:16647722-Estrogen Replacement Therapy, pubmed-meshheading:16647722-Estrogens, pubmed-meshheading:16647722-Female, pubmed-meshheading:16647722-Heart, pubmed-meshheading:16647722-Heart Ventricles, pubmed-meshheading:16647722-Homeostasis, pubmed-meshheading:16647722-Membrane Proteins, pubmed-meshheading:16647722-Muscle Proteins, pubmed-meshheading:16647722-Myocardial Contraction, pubmed-meshheading:16647722-Organ Size, pubmed-meshheading:16647722-Ovariectomy, pubmed-meshheading:16647722-Papillary Muscles, pubmed-meshheading:16647722-RNA, Messenger, pubmed-meshheading:16647722-Rats, pubmed-meshheading:16647722-Rats, Sprague-Dawley, pubmed-meshheading:16647722-Receptors, Adrenergic, beta, pubmed-meshheading:16647722-Sodium-Calcium Exchanger
pubmed:year
2006
pubmed:articleTitle
Effect of estrogen on calcium-handling proteins, beta-adrenergic receptors, and function in rat heart.
pubmed:affiliation
Department of Clinical Nursing Science, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't