Linked Life Data

16647292

Source:http://linkedlifedata.com/resource/pubmed/id/16647292

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2006-5-31
pubmed:abstractText
A 1.5-kb genomic DNA fragment corresponding to the 5'-flanking region of the rat LDL receptor gene was cloned and putative regulatory regions were identified. A major transcription start site was identified at -154 bp relative to the ATG translation initiation codon, within a region containing two thyroid hormone response element half-site motifs (2H-TRE). Binding of thyroid hormone receptors alpha and beta1 to this element was demonstrated. Mutations within this 2H-TRE region abolished basal transcription levels of the rat LDL receptor gene. Reporter gene studies indicated that the promoter region between -300 and -200 bp, which contains one sterol response element (SRE) and two specificity protein-1 sites (Sp1) sites, is crucial for basal transcription of the rat LDL receptor gene. The functionality of the SRE motif was confirmed using electrophoretic mobility shift assays and reporter gene studies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
1761
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
492-500
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Characterization of the rat LDL receptor 5'-flanking region.
pubmed:affiliation
Department of Biochemistry and Molecular Biology College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA. dlopez@hsc.usf.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't