Linked Life Data

16641260

Source:http://linkedlifedata.com/resource/pubmed/id/16641260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2006-4-27
pubmed:abstractText
APOBEC3G (APO3G) is a cytidine deaminase that restricts replication of vif-defective human immunodeficiency virus type 1 (HIV-1). Like other members of the cellular deaminase family, APO3G has the propensity to form homo-multimers. In the current study, we investigated the functional determinants for multimerization of human APO3G and studied the role of APO3G multimerization for catalytic activity, virus encapsidation, and antiviral activity. We found that human APO3G is capable of forming multimeric complexes in transfected HeLa cells. Interestingly, multimerization of APO3G was exquisitely sensitive to RNase treatment, suggesting that interaction of APO3G subunits is facilitated or stabilized by an RNA bridge. Mutation of a conserved cysteine residue (C97) that is part of an N-terminal zinc-finger motif in APO3G abolished multimerization of APO3G; however, the C97 mutation inhibited neither in vitro deaminase activity nor antiviral function of APO3G. These results suggest that monomeric APO3G is both catalytically active and has antiviral activity. Interference studies employing either catalytically inactive or packaging-incompetent APO3G variants suggest that wild-type APO3G is packaged into HIV-1 particles in monomeric form. These results provide novel insights into the catalytic function and antiviral property of APO3G and demonstrate an important role for C97 in the RNA-dependent multimerization of this protein.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-10704474, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-10915601, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-11863358, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12683974, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12750511, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12756266, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12808465, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12808466, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12809610, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12859895, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12910268, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-12970355, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14527406, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14528300, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14528301, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14557625, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14564014, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14614829, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14672928, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14722068, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-14747572, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15031497, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15098018, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15148397, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15152192, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15156567, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15159405, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15210704, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15215254, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15358144, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15464836, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15479826, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15496550, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15516966, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15668174, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15721369, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15827203, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-15829920, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-16082223, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-7782342, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-7782343, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-8078915, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-8523563, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-8999814, http://linkedlifedata.com/resource/pubmed/commentcorrection/16641260-9466941
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4673-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Monomeric APOBEC3G is catalytically active and has antiviral activity.
pubmed:affiliation
Laboratory of Molecular Microbiology, Viral Biochemistry Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0460, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Intramural