Linked Life Data

16641145

Source:http://linkedlifedata.com/resource/pubmed/id/16641145

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-8-24
pubmed:abstractText
In Sertoli cells of testis, androgen receptor-regulated gene transcription plays an indispensable role in maintaining spermatogenesis. Androgen receptor activity is modulated by a number of coregulators which are associated with the androgen receptor. Non-POU-domain-containing, octamer binding protein (NONO), a member of the DBHS-containing proteins, complexes with androgen receptor and functions as a coactivator for the receptor. Paraspeckle protein 1 alpha isoform (PSPC1, previously known as PSP1) and Splicing factor, proline- and glutamine-rich (SFPQ, previously known as PSF), other members of the DBHS-containing proteins, are also found in androgen receptor complexes, suggesting that these DBHS-containing proteins may cooperatively regulate androgen receptor-mediated gene transcription. We demonstrated that PSPC1, NONO, and SFPQ are coexpressed in Sertoli cell line TTE3 and interact reciprocally. The effect of the DBHS-containing proteins on the transcriptional activity was assessed using the construct containing androgen-responsive elements followed by a luciferase gene. The results showed that all the DBHS-containing proteins activate androgen receptor-mediated transcription, and PSPC1 is the most effective coactivator among them. Furthermore, we confirmed the presence of PSPC1, NONO, and SFPQ proteins in Sertoli cells of adult mouse testis sections. These observations suggest that PSPC1, NONO, and SFPQ form complexes with each other in Sertoli cells and may regulate androgen receptor-mediated transcriptional activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
352-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16641145-Animals, pubmed-meshheading:16641145-Antibodies, Monoclonal, pubmed-meshheading:16641145-Blotting, Western, pubmed-meshheading:16641145-Cell Line, pubmed-meshheading:16641145-DNA-Binding Proteins, pubmed-meshheading:16641145-Genes, Reporter, pubmed-meshheading:16641145-Immunohistochemistry, pubmed-meshheading:16641145-Immunoprecipitation, pubmed-meshheading:16641145-Luciferases, pubmed-meshheading:16641145-Male, pubmed-meshheading:16641145-Mice, pubmed-meshheading:16641145-Nuclear Proteins, pubmed-meshheading:16641145-Plasmids, pubmed-meshheading:16641145-Polypyrimidine Tract-Binding Protein, pubmed-meshheading:16641145-RNA-Binding Proteins, pubmed-meshheading:16641145-Receptors, Androgen, pubmed-meshheading:16641145-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16641145-Sertoli Cells, pubmed-meshheading:16641145-Testis, pubmed-meshheading:16641145-Transcription, Genetic, pubmed-meshheading:16641145-Transcriptional Activation
pubmed:year
2006
pubmed:articleTitle
PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription.
pubmed:affiliation
Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama 240-8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't