Source:http://linkedlifedata.com/resource/pubmed/id/16638019
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2006-5-15
|
| pubmed:abstractText |
We studied the lysophosphatidic acid receptor-1 (LPA1) gene, which we found to be expressed endogenously in cultured hippocampal neurons, and in vivo in young (1-week-old) rat brain slices. Overexpressed green fluorescent protein (GFP)-tagged, membrane-associated LPA1 accumulated in a punctate manner over the entire dendritic tree and caused an increase in dendritic spine density. About half of the dendritic spines in the LPA1-transfected neurons displayed distinct fluorescent puncta, and this subset of spines was also substantially larger than puncta-free, LPA1-transfected or control GFP spines. This phenotype could also be seen in cells transfected with a ligand-binding, defective mutant and is therefore not dependent on interaction with an ambient ligand. While spontaneous miniature excitatory synaptic currents were of the same amplitudes, they decayed slower in LPA1-transfected neurons compared with GFP controls. We propose that LPA1 may play a role in the formation and modulation of the dendritic spine synapse.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
97
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1379-92
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16638019-Animals,
pubmed-meshheading:16638019-Animals, Newborn,
pubmed-meshheading:16638019-Binding Sites,
pubmed-meshheading:16638019-Cell Differentiation,
pubmed-meshheading:16638019-Cells, Cultured,
pubmed-meshheading:16638019-Dendritic Spines,
pubmed-meshheading:16638019-Excitatory Postsynaptic Potentials,
pubmed-meshheading:16638019-Green Fluorescent Proteins,
pubmed-meshheading:16638019-Hippocampus,
pubmed-meshheading:16638019-Mutation,
pubmed-meshheading:16638019-Neurons,
pubmed-meshheading:16638019-Organ Culture Techniques,
pubmed-meshheading:16638019-Protein Binding,
pubmed-meshheading:16638019-Rats,
pubmed-meshheading:16638019-Receptors, Lysophosphatidic Acid,
pubmed-meshheading:16638019-Synapses,
pubmed-meshheading:16638019-Synaptic Transmission,
pubmed-meshheading:16638019-Transfection
|
| pubmed:year |
2006
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| pubmed:articleTitle |
The role of LPA1 in formation of synapses among cultured hippocampal neurons.
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| pubmed:affiliation |
Department of Neurobiology, The Weizmann Institute, Rehovot, Israel. yair.pilpel@mpimf-deidelberg.mpg.de
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|