Linked Life Data

16637561

Source:http://linkedlifedata.com/resource/pubmed/id/16637561

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-4-26
pubmed:abstractText
The need for a rapid method by which first responders can screen for the presence of spores in powder samples has been increased since the anthrax attack of 2001. The majority of powders that were sampled in the context of that attack were hoaxes and did not contain Bacillus anthracis. The large number of samples overwhelmed the analysis capacity of public health laboratories. A rapid screening method for determining the presence of viable spores would eliminate much laboratory work and expedite procedures for identification if spores were detected. In the study reported here, Bacillus thuringiensis was used as a surrogate for B. anthracis to investigate if heat shock followed by luminescence analysis would allow rapid quantification of viable spores. The aim of the study was to investigate the best and fastest heat shock conditions that would trigger the breakdown of endospore dormancy of Bacillus species and give a higher luminescence signal. Heat shock and luminescence do not identify the type of spores in a powder sample, but they can detect the presence of viable spores in near real time. Different types of germinant were tested at different conditions (germination duration, temperature, concentration). The rapid method was compared with the traditional plate count method. The results showed that the rapid method can be used as a rapid (< 15 min), sensitive (< 100 spores), and inexpensive detection technique. The rapid method can be applied on site when suspicious powder is found and enables decision making for further identification.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-0892
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
34-7, 44, 46
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Simple and rapid method for detection of bacterial spores in powder useful for first responders.
pubmed:affiliation
University of Michigan, Department of Environmental Health Sciences, Ann Arbor 48109-2029, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't