Linked Life Data

16635248

Source:http://linkedlifedata.com/resource/pubmed/id/16635248

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2006-4-25
pubmed:abstractText
Neurotransmitters and their receptors have been involved in both proper brain development and neurodevelopmental disorders. The role that nicotinic receptors play in immature cortical neurons was initially investigated by gene profiling using Affymetrix DNA arrays. Both short (15 min) and prolonged (18 h) treatments with nicotine did not induce modification in gene expression, whereas a significant down-regulation of c-fos protein levels was observed after 18 h treatment. Conversely, a brief treatment with the glutamatergic agonist NMDA triggered up-regulation of immediate early genes and transcription factors, which remained unaffected by pre-treatment for 18 h with nicotine. Calcium imaging studies revealed that NMDA activated a sustained increase in intracellular calcium concentration in the majority of neurons, whereas nicotine evoked only a transient calcium increase in a smaller percentage of neurons, suggesting that the calcium signalling response was correlated with activation of gene expression. Nicotine effects on immature cortical neurons perhaps do not require gene regulation but may be still acting on signalling pathways.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
97 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-43
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16635248-Animals, pubmed-meshheading:16635248-Calcium, pubmed-meshheading:16635248-Cells, Cultured, pubmed-meshheading:16635248-Cerebral Cortex, pubmed-meshheading:16635248-Embryo, Mammalian, pubmed-meshheading:16635248-Gene Expression Regulation, Developmental, pubmed-meshheading:16635248-N-Methylaspartate, pubmed-meshheading:16635248-Neurons, pubmed-meshheading:16635248-Neurotransmitter Agents, pubmed-meshheading:16635248-Nicotine, pubmed-meshheading:16635248-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:16635248-Proto-Oncogene Proteins c-fos, pubmed-meshheading:16635248-Rats, pubmed-meshheading:16635248-Receptors, Cholinergic, pubmed-meshheading:16635248-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:16635248-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16635248-Signal Transduction
pubmed:year
2006
pubmed:articleTitle
Gene expression is differentially regulated by neurotransmitters in embryonic neuronal cortical culture.
pubmed:affiliation
Mental Retardation Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-7332, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural