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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-4-25
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pubmed:abstractText |
MG7-Ag, gastric cancer-associated antigen, has been shown to be immunogenic and has been used as marker molecule for prognosis. In a previous study, we developed an oral DNA vaccine based on MG7-Ag mimotope. However, we failed to detect cellular immune response using the oral MG7-Ag mimotope DNA vaccine. To induce significant T cell response, we developed a recombinant adenovirus vaccine based on MG7-Ag mimotope and evaluated the efficacy and protective effects of heterologous prime-boost immunization protocol with an oral DNA vaccine previously developed. We found that both vaccines were able to elicit a significant humoral response against MG7-Ag, while the highest serum titre MG7 antibody was detected in mice immunized with the heterologous prime-boost immunization protocol. Enzyme-linked immunospot (ELISPOT) assay demonstrated that the heterologous prime-boost immunization strategy was more efficient in inducing T cell response than the homologous prime-boost strategy. In the tumour challenge assay, 2 of 5 mice immunized with the heterologous prime-boost protocol were tumour free, while none of the mice in homologous prime-boost groups or control groups was tumour free. Those tumour-bearing mice in the heterologous prime-boost regime had smaller tumour masses than their counterparts in the homologous prime-boost groups or control groups. Therefore, our study suggests that vaccines against MG7-Ag induce significant immune response against gastric cancer, and that the heterologous prime-boost protocol using different types of vaccines could achieve better protective effect than the homologous prime-boost protocol.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-10640958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-11803063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-12018818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-12477888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-12639484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-12657725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-12800222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-1370083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15102369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15155102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15308363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15507315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15724244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15740477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15759317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-15876863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-7895164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-9032353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-9413986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-9482916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-9546783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16634806-9787153
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/MG7 antigen, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0009-9104
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pubmed:author |
pubmed-author:ChenYY,
pubmed-author:DingJJ,
pubmed-author:FauMM,
pubmed-author:GutDD,
pubmed-author:HamPP,
pubmed-author:LiangSS,
pubmed-author:LimRR,
pubmed-author:LinTT,
pubmed-author:MensJJ,
pubmed-author:SunLL,
pubmed-author:XiaLL,
pubmed-author:YuZZ
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pubmed:issnType |
Print
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pubmed:volume |
144
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
319-25
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pubmed:dateRevised |
2010-9-16
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pubmed:meshHeading |
pubmed-meshheading:16634806-Adenoviridae,
pubmed-meshheading:16634806-Administration, Oral,
pubmed-meshheading:16634806-Animals,
pubmed-meshheading:16634806-Antibodies, Neoplasm,
pubmed-meshheading:16634806-Antibody Formation,
pubmed-meshheading:16634806-Antigens, Neoplasm,
pubmed-meshheading:16634806-Cancer Vaccines,
pubmed-meshheading:16634806-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16634806-Female,
pubmed-meshheading:16634806-Immunity, Cellular,
pubmed-meshheading:16634806-Interferon-gamma,
pubmed-meshheading:16634806-Mice,
pubmed-meshheading:16634806-Mice, Inbred BALB C,
pubmed-meshheading:16634806-Stomach Neoplasms,
pubmed-meshheading:16634806-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:16634806-Vaccines, DNA,
pubmed-meshheading:16634806-Vaccines, Synthetic,
pubmed-meshheading:16634806-Viral Vaccines
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pubmed:year |
2006
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pubmed:articleTitle |
Enhanced immunogenicity and antitumour effects with heterologous prime-boost regime using vaccines based on MG7-Ag mimotope of gastric cancer.
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pubmed:affiliation |
State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, 15 Changle West Road, Xi'an 710032, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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