Linked Life Data

16631141

Source:http://linkedlifedata.com/resource/pubmed/id/16631141

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-5-29
pubmed:abstractText
Two novel granin-like polypeptides, VGF and pro-SAAS, which are stored in and released from secretory vesicles and are expressed widely in nervous, endocrine, and neuroendocrine tissues, play roles in the regulation of body weight, feeding, and energy expenditure. Both VGF and pro-SAAS are cleaved into peptide fragments, several of which are biologically active. We utilized a highly sensitive and specific radioimmunoassay (RIA) to immunoreactive, pro-SAAS-derived PEN peptides, developed another against immunoreactive, VGF-derived AQEE30 peptides, and quantified these peptides in various mouse tissues and brain regions. Immunoreactive AQEE30 was most abundant in the pituitary, while brain levels were highest in hypothalamus, striatum, and frontal cortex. Immunoreactive PEN levels were highest in the pancreas and spinal cord, and in brain, PEN was most abundant in striatum, hippocampus, pons and medulla, and cortex. Since both peptides were expressed in hypothalamus, a region of the brain that controls feeding and energy expenditure, double label immunofluorescence studies were employed. These demonstrated that 42% of hypothalamic arcuate neurons coexpress VGF and SAAS peptides, and that the intracellular distributions of these peptides in arcuate neurons differed. By RIA, cold stress increased immunoreactive AQEE30 and PEN peptide levels in female but not male hypothalamus, while a high fat diet increased AQEE30 and PEN peptide levels in female but not male hippocampus. VGF and SAAS-derived peptides are therefore widely expressed in endocrine, neuroendocrine, and neural tissues, can be accurately quantified by RIA, and are differentially regulated in the brain by diet and cold stress.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
1089
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-32
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16631141-Animals, pubmed-meshheading:16631141-Brain, pubmed-meshheading:16631141-Cold Temperature, pubmed-meshheading:16631141-Dietary Fats, pubmed-meshheading:16631141-Disease Models, Animal, pubmed-meshheading:16631141-Energy Intake, pubmed-meshheading:16631141-Female, pubmed-meshheading:16631141-Food, Formulated, pubmed-meshheading:16631141-Food Habits, pubmed-meshheading:16631141-Hippocampus, pubmed-meshheading:16631141-Hypothalamus, pubmed-meshheading:16631141-Male, pubmed-meshheading:16631141-Mice, pubmed-meshheading:16631141-Mice, Inbred C57BL, pubmed-meshheading:16631141-Nerve Tissue Proteins, pubmed-meshheading:16631141-Neurons, pubmed-meshheading:16631141-Neuropeptides, pubmed-meshheading:16631141-Organ Specificity, pubmed-meshheading:16631141-Pancreas, pubmed-meshheading:16631141-Peptides, pubmed-meshheading:16631141-Radioimmunoassay, pubmed-meshheading:16631141-Sex Characteristics, pubmed-meshheading:16631141-Stress, Physiological, pubmed-meshheading:16631141-Up-Regulation
pubmed:year
2006
pubmed:articleTitle
Quantification of VGF- and pro-SAAS-derived peptides in endocrine tissues and the brain, and their regulation by diet and cold stress.
pubmed:affiliation
Fishberg Department of Neuroscience, Mount Sinai School of Medicine, New York, NY 10029, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural