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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-4-24
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pubmed:abstractText |
Polycomb group proteins are essential for early development in metazoans, but their contributions to human development are not well understood. We have mapped the Polycomb Repressive Complex 2 (PRC2) subunit SUZ12 across the entire nonrepeat portion of the genome in human embryonic stem (ES) cells. We found that SUZ12 is distributed across large portions of over two hundred genes encoding key developmental regulators. These genes are occupied by nucleosomes trimethylated at histone H3K27, are transcriptionally repressed, and contain some of the most highly conserved noncoding elements in the genome. We found that PRC2 target genes are preferentially activated during ES cell differentiation and that the ES cell regulators OCT4, SOX2, and NANOG cooccupy a significant subset of these genes. These results indicate that PRC2 occupies a special set of developmental genes in ES cells that must be repressed to maintain pluripotency and that are poised for activation during ES cell differentiation.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:AbeKuniyaK,
pubmed-author:BartelDavid PDP,
pubmed-author:BellGeorge WGW,
pubmed-author:BoyerLaurie ALA,
pubmed-author:ChevalierBrettB,
pubmed-author:ColeMegan FMF,
pubmed-author:FuchikamiTakuyaT,
pubmed-author:GiffordDavid KDK,
pubmed-author:GuentherMatthew GMG,
pubmed-author:HannettNancy MNM,
pubmed-author:HerbolsheimerElizabethE,
pubmed-author:IsonoKyo-ichiK,
pubmed-author:JaenischRudolfR,
pubmed-author:JennerRichard GRG,
pubmed-author:JohnstoneSarah ESE,
pubmed-author:KosekiHaruhikoH,
pubmed-author:KumarRoshan MRM,
pubmed-author:LeeTong IhnTI,
pubmed-author:LevineStuart SSS,
pubmed-author:MeltonDouglas ADA,
pubmed-author:MurrayHeather LHL,
pubmed-author:OdomDuncan TDT,
pubmed-author:OtteArie PAP,
pubmed-author:SunKaimingK,
pubmed-author:VolkertThomas LTL,
pubmed-author:YoungRichard ARA,
pubmed-author:YuanBingbingB,
pubmed-author:ZuckerJacob PJP
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
301-13
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pubmed:dateRevised |
2010-8-23
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pubmed:meshHeading |
pubmed-meshheading:16630818-Animals,
pubmed-meshheading:16630818-Carrier Proteins,
pubmed-meshheading:16630818-Cells, Cultured,
pubmed-meshheading:16630818-Gene Expression Profiling,
pubmed-meshheading:16630818-Gene Expression Regulation, Developmental,
pubmed-meshheading:16630818-Humans,
pubmed-meshheading:16630818-Multiprotein Complexes,
pubmed-meshheading:16630818-Nuclear Proteins,
pubmed-meshheading:16630818-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16630818-Protein Subunits,
pubmed-meshheading:16630818-RNA Polymerase II,
pubmed-meshheading:16630818-Signal Transduction,
pubmed-meshheading:16630818-Stem Cells,
pubmed-meshheading:16630818-Transcription, Genetic,
pubmed-meshheading:16630818-Transcription Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Control of developmental regulators by Polycomb in human embryonic stem cells.
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pubmed:affiliation |
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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