Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-7-26
pubmed:abstractText
Saccharomyces mating-type (MAT) switching occurs by gene conversion using one of two donors, HMLalpha and HMRa, located near the ends of the same chromosome. MATa cells preferentially choose HMLalpha, a decision that depends on the recombination enhancer (RE) that controls recombination along the left arm of chromosome III (III-L). When RE is inactive, the two chromosome arms constitute separate domains inaccessible to each other; thus HMRa, located on the same arm as MAT, becomes the default donor. Activation of RE increases HMLalpha usage, even when RE is moved 50 kb closer to the centromere. If MAT is inserted into the same domain as HML, RE plays little or no role in activating HML, thus ruling out any role for RE in remodeling the silent chromatin of HML in regulating donor preference. When the donors MAT and RE are moved to chromosome V, RE increases HML usage, but the inaccessibility of HML without RE apparently depends on other chromosome III-specific sequences. Similar conclusions were reached when RE was placed adjacent to leu2 or arg4 sequences engaged in spontaneous recombination. We propose that RE's targets are anchor sites that tether chromosome III-L in MATalpha cells thus reducing its mobility in the nucleus.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-10514571, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-10567520, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-12183363, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-12748633, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-14527325, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-1459444, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-14631041, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-14745000, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-15082540, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-15568979, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-16809780, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-1735128, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-2684745, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-3323810, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-3549449, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-6280875, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-6287218, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-7042099, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-7649475, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-7747518, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-8209257, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-8242745, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-8552094, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-8553698, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-8861911, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-8904338, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-9250679, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-9335581, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-9620858, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-9708741, http://linkedlifedata.com/resource/pubmed/commentcorrection/16624909-9710623
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1197-206
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Saccharomyces cerevisiae donor preference during mating-type switching is dependent on chromosome architecture and organization.
pubmed:affiliation
Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural