Source:http://linkedlifedata.com/resource/pubmed/id/16624804
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
25
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pubmed:dateCreated |
2006-6-19
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pubmed:databankReference | |
pubmed:abstractText |
Interleukin enhancer binding factor (ILF) is a human transcription factor and a new member of the winged helix/forkhead family. ILF can bind to purine-rich regulatory motifs such as the human T-cell leukemia virus-long terminal region and the interleukin-2 promoter. Here we report the 2.4 A crystal structure of two DNA binding domains of ILF (FOXK1a) binding to a 16-bp DNA duplex containing a promoter sequence. Electrophoretic mobility shift assay studies demonstrate that two ILF-DNA binding domain molecules cooperatively bind to DNA. In addition to the recognition helix recognizing the core sequences through the major groove, the structure shows that wing 1 interacts with the minor groove of DNA, and the H2-H3 loop region makes ionic bonds to the phosphate group, which permits the recognition of DNA. The structure also reveals that the presence of the C-terminal alpha-helix in place of a typical wing 2 in a member of this family alters the orientation of the C-terminal basic residues (RKRRPR) when binding to DNA outside the core sequence. These results provide a new insight into how the DNA binding specificities of winged helix/forkhead proteins may be regulated by their less conserved regions.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17400-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16624804-Amino Acid Sequence,
pubmed-meshheading:16624804-Base Pairing,
pubmed-meshheading:16624804-Crystallography, X-Ray,
pubmed-meshheading:16624804-Forkhead Transcription Factors,
pubmed-meshheading:16624804-Humans,
pubmed-meshheading:16624804-Models, Molecular,
pubmed-meshheading:16624804-Molecular Conformation,
pubmed-meshheading:16624804-Molecular Sequence Data,
pubmed-meshheading:16624804-Nucleic Acid Conformation,
pubmed-meshheading:16624804-Promoter Regions, Genetic,
pubmed-meshheading:16624804-Protein Binding,
pubmed-meshheading:16624804-Protein Structure, Secondary,
pubmed-meshheading:16624804-Protein Structure, Tertiary,
pubmed-meshheading:16624804-Sequence Homology, Amino Acid
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pubmed:year |
2006
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pubmed:articleTitle |
Crystal structure of the human FOXK1a-DNA complex and its implications on the diverse binding specificity of winged helix/forkhead proteins.
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pubmed:affiliation |
Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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