Source:http://linkedlifedata.com/resource/pubmed/id/16622419
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
2006-5-12
|
pubmed:abstractText |
The basic element for chromosome inheritance, the centromere, is epigenetically determined in mammals. The prime candidate for specifying centromere identity is the array of nucleosomes assembled with CENP-A, the centromere-specific histone H3 variant. Here, we show that CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of three new human centromere proteins (CENP-M, CENP-N and CENP-T), along with CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Facilitates chromatin transcription (FACT) and nucleophosmin-1 (previously implicated in transcriptional chromatin remodelling and as a multifunctional nuclear chaperone, respectively) are absent from histone H3-containing nucleosomes, but are stably recruited to CENP-A nucleosomes independent of CENP-A NAC. Seven new CENP-A-nucleosome distal (CAD) centromere components (CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S) are identified as assembling on the CENP-A NAC. The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint signalling.
|
pubmed:grant | |
pubmed:commentsCorrections | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin Assembly Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleosomes,
http://linkedlifedata.com/resource/pubmed/chemical/centromere protein A
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
1465-7392
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
8
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
458-69
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:16622419-Amino Acid Sequence,
pubmed-meshheading:16622419-Autoantigens,
pubmed-meshheading:16622419-Centromere,
pubmed-meshheading:16622419-Chromatin Assembly Factor-1,
pubmed-meshheading:16622419-Chromatin Assembly and Disassembly,
pubmed-meshheading:16622419-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:16622419-Chromosomes, Human,
pubmed-meshheading:16622419-DNA-Binding Proteins,
pubmed-meshheading:16622419-HeLa Cells,
pubmed-meshheading:16622419-Histones,
pubmed-meshheading:16622419-Humans,
pubmed-meshheading:16622419-Mitosis,
pubmed-meshheading:16622419-Molecular Sequence Data,
pubmed-meshheading:16622419-Nucleosomes,
pubmed-meshheading:16622419-Protein Binding,
pubmed-meshheading:16622419-Signal Transduction
|
pubmed:year |
2006
|
pubmed:articleTitle |
The human CENP-A centromeric nucleosome-associated complex.
|
pubmed:affiliation |
Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093-0670, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|