Source:http://linkedlifedata.com/resource/pubmed/id/16622059
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 5
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| pubmed:dateCreated |
2006-4-19
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| pubmed:abstractText |
The long-chain acyl-CoA synthase (ACS) FadD1 plays an important role in timing the levels of antibiotic production in Streptomyces coelicolor. fadD1 and macs1, encoding a putative medium-chain ACS, are part of a two-gene operon, whose expression is induced during the stationary phase of growth. Here it is reported that transcription of the macs1-fadD1 operon is positively regulated by AcsR, a LuxR-type transcriptional regulator. In an acsR mutant, expression of the macs1-fadD1 genes loses its normal up-regulation and the mutant becomes deficient in antibiotic production, in a clear correlation with the phenotype shown by a fadD1 null mutant. The absence of macs1-fadD1 induction in the acsR mutant was restored by complementation with a wild-type copy of the acsR gene, showing a strict link between AcsR and induction of the macs1-fadD1 operon. Gel mobility shift assays and DNase I footprinting indicated that AcsR binds to specific sequences about +162 nucleotides downstream of the macs1 transcriptional start site. In the putative operator sequence three almost identical direct tandem repeats of seven nucleotides were identified where the central sequence is essential for AcsR recognition and binding. Transcriptional fusions of the divergent pacsR and pmacs1 promoters indicated that AcsR does not regulate its own transcription, and that it binds to the operator region to control exclusively the growth-phase induction of the macs1-fadD1 operon.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Catechol 2,3-Dioxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Coenzyme A Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1350-0872
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
152
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1427-39
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:16622059-Amino Acid Sequence,
pubmed-meshheading:16622059-Anti-Bacterial Agents,
pubmed-meshheading:16622059-Artificial Gene Fusion,
pubmed-meshheading:16622059-Base Sequence,
pubmed-meshheading:16622059-Catechol 2,3-Dioxygenase,
pubmed-meshheading:16622059-Coenzyme A Ligases,
pubmed-meshheading:16622059-DNA Footprinting,
pubmed-meshheading:16622059-DNA-Binding Proteins,
pubmed-meshheading:16622059-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16622059-Gene Expression Regulation, Bacterial,
pubmed-meshheading:16622059-Genes, Regulator,
pubmed-meshheading:16622059-Genetic Complementation Test,
pubmed-meshheading:16622059-Luciferases,
pubmed-meshheading:16622059-Molecular Sequence Data,
pubmed-meshheading:16622059-Mutation,
pubmed-meshheading:16622059-Operator Regions, Genetic,
pubmed-meshheading:16622059-Operon,
pubmed-meshheading:16622059-Promoter Regions, Genetic,
pubmed-meshheading:16622059-Protein Binding,
pubmed-meshheading:16622059-Streptomyces coelicolor,
pubmed-meshheading:16622059-Transcription, Genetic
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| pubmed:year |
2006
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| pubmed:articleTitle |
Transcriptional regulation of the macs1-fadD1 operon encoding two acyl-CoA synthases involved in the physiological differentiation of Streptomyces coelicolor.
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| pubmed:affiliation |
Microbiology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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