Source:http://linkedlifedata.com/resource/pubmed/id/16621600
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2006-10-9
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pubmed:abstractText |
The AAA family proteins usually form a hexameric ring structure. The ATP-binding pocket, which is located at the interface of subunits in the hexamer, consists of three functionally important motifs, the Walker A and B motifs, and the second region of homology (SRH). It is well known that Walker A and B motifs mediate ATP binding and hydrolysis, respectively. Highly conserved arginine residues in the SRH have been proposed to function as arginine fingers, which interact with the gamma-phosphate of bound ATP. To elucidate the mechanism of ATP hydrolysis, we prepared several mutants of the Caenorhabditis elegans fidgetin homologue FIGL-1 carrying a mutation in each of the above-mentioned three motifs. None of the constructed mutants showed ATPase activity. All the mutants except for K362A were able to bind ATP. A decrease in the ATPase activity by mixing wild-type and each mutant subunits was caused by the formation of hetero-hexamers. Mixtures of E416A and R471A, or N461A and R471A led to the formation of hetero-hexamers with partially restored ATPase activities, providing direct, firm evidence for the intersubunit catalysis model. In addition, based on the results obtained with mixtures of K362A with wild-type or R471A subunits, we propose that a conformational change upon ATP binding is required for proper orientation of the arginine fingers, which is essential for efficient hydrolysis of ATP bound to the neighboring subunit.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/F32D1.1 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/fidgetin protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1047-8477
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
156
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-100
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pubmed:meshHeading |
pubmed-meshheading:16621600-Adenosine Triphosphatases,
pubmed-meshheading:16621600-Amino Acid Motifs,
pubmed-meshheading:16621600-Amino Acid Substitution,
pubmed-meshheading:16621600-Animals,
pubmed-meshheading:16621600-Arginine,
pubmed-meshheading:16621600-Caenorhabditis elegans Proteins,
pubmed-meshheading:16621600-Catalysis,
pubmed-meshheading:16621600-Hydrolysis,
pubmed-meshheading:16621600-Models, Biological,
pubmed-meshheading:16621600-Models, Molecular,
pubmed-meshheading:16621600-Nuclear Proteins,
pubmed-meshheading:16621600-Protein Structure, Tertiary,
pubmed-meshheading:16621600-Protein Subunits,
pubmed-meshheading:16621600-Spodoptera
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pubmed:year |
2006
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pubmed:articleTitle |
Mutational analysis of the functional motifs in the ATPase domain of Caenorhabditis elegans fidgetin homologue FIGL-1: firm evidence for an intersubunit catalysis mechanism of ATP hydrolysis by AAA ATPases.
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pubmed:affiliation |
Division of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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